Cancer Cell Internalization of Gold Nanostars Impacts Their Photothermal Efficiency In Vitro and In Vivo: Toward a Plasmonic Thermal Fingerprint in Tumoral Environment
Cancer Cell Internalization of Gold Nanostars Impacts Their Photothermal Efficiency In Vitro and In Vivo: Toward a Plasmonic Thermal Fingerprint in Tumoral Environment
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DOI：
10.1002/adhm.201501035
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发表时间：
2016-05-11
影响因子：
10
通讯作者：
Wilhelm, Claire
中科院分区：
文献类型：
--
作者：
Espinosa, Ana;Silva, Amanda K. A.;Wilhelm, Claire
金纳米粒子是癌症热疗的主要候选者。然而，虽然纳米粒子介导的光热疗法的最终目标是癌细胞，但以前没有在肿瘤环境中评估加热性能。本文对金纳米星原位产热效率进行了系统的研究：不仅在体外癌细胞中，而且在体内瘤内注射后。它表明，（i）在水性分散体中，热生成是由颗粒大小和激发激光波长;（ii）在体外的癌细胞中，热生成仍然是非常有效的，但无论颗粒大小和激光波长;和（iii）热生成的nanostars注射到体内肿瘤随时间的推移而演变，因为nanostars从细胞外基质运输到内涵体。因此，等离子体激元加热响应作为细胞中纳米颗粒内化的特征，使纳米颗粒介导的光热治疗的最终目标更近了一步。
Gold nanoparticles are prime candidates for cancer thermotherapy. However, while the ultimate target for nanoparticle-mediated photothermal therapy is the cancer cell, heating performance has not previously been evaluated in the tumoral environment. A systematic investigation of gold nanostar heat-generating efficiency in situ is presented: not only in cancer cells in vitro but also after intratumoral injection in vivo. It is demonstrated that (i) in aqueous dispersion, heat generation is governed by particle size and exciting laser wavelength; (ii) in cancer cells in vitro, heat generation is still very efficient, but irrespective of both particle size and laser wavelength; and (iii) heat generation by nanostars injected into tumors in vivo evolves with time, as the nanostars are trafficked from the extracellular matrix into endosomes. The plasmonic heating response thus serves as a signature of nanoparticle internalization in cells, bringing the ultimate goal of nanoparticle-mediated photothermal therapy a step closer.