Reduced expression of SP1 and SP4 transcription factors in peripheral blood mononuclear cells in first-episode psychosis
Reduced expression of SP1 and SP4 transcription factors in peripheral blood mononuclear cells in first-episode psychosis
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DOI：
10.1016/j.jpsychires.2013.07.019
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发表时间：
2013-11-01
影响因子：
4.8
通讯作者：
Ramos, Belen
中科院分区：
文献类型：
--
作者：
Fuste, Montserrat;Pinacho, Raquel;Ramos, Belen
转录因子特异性蛋白4（SP4）和1（SP1）的改变与不同的神经精神疾病有关。前额叶皮层中SP4和SP1蛋白水平的降低分别与双相情感障碍和精神分裂症相关，这表明这两种因素都可能参与具有精神病特征的疾病的发病机制。本研究的目的是调查是否减少SP1，SP4和SP3蛋白和mRNA表达的外周血单个核细胞在精神病的早期阶段可能作为这些疾病的一个潜在的生物标志物。设计了一项对首发精神病患者（n = 14）与性别和年龄匹配的健康对照组（n = 14）进行比较的横断面研究。通过Parc Sanitari Sant Joan de Deu的成人心理健康服务招募患者。对首发精神病患者和健康对照者外周血单个核细胞中SP1、SP4和SP3的蛋白和基因表达水平进行了评估。我们报告说，SP1和SP4的蛋白质水平，但不是SP3，显着降低患者相比，对照组。相反，我们没有观察到患者组和对照组之间SP1、SP4或SP3基因的表达水平有任何差异。在患者中，SP4蛋白水平与SP1蛋白水平显著相关。然而，没有发现每个因子的蛋白质和基因表达水平之间的关联。我们的研究表明，在淋巴细胞中首次发作精神病的SP1和SP4蛋白水平降低，这表明这些转录因子是早期精神病谱系障碍的潜在外周生物标志物。(C)2013爱思唯尔有限公司版权所有。
Alterations of transcription factor specificity protein 4 (SP4) and 1 (SP1) have been linked to different neuropsychiatric diseases. Reduced SP4 and SP1 protein levels in the prefrontal cortex have been associated with bipolar disorder and schizophrenia, respectively, suggesting that both factors could be involved in the pathogenesis of disorders with psychotic features. The aim of this study was to investigate whether the reduction of SP1, SP4 and SP3 protein and mRNA expression in peripheral blood mononuclear cells in the early stages of psychosis may act as a potential biomarker of these disorders. A cross-sectional study of first-episode psychosis patients (n = 14) compared to gender- and age-matched healthy controls (n = 14) was designed. Patients were recruited through the adult mental health services of Parc Sanitari Sant Joan de Deu. Protein and gene expression levels of SP1, SP4 and SP3 were assessed in peripheral blood mononuclear cells of patients with first-episode psychosis and healthy control subjects. We report that protein levels of SP1 and SP4, but not SP3, are significantly reduced in patients compared to controls. In contrast, we did not observe any differences in expression levels for SP1, SP4 or SP3 genes between patient and control groups. In patients, SP4 protein levels were significantly associated with SP1 protein levels. No association was found, however, between protein and gene expression levels for each factor. Our study shows reduced SP1 and SP4 protein levels in first-episode psychosis in lymphocytes, suggesting that these transcription factors are potential peripheral biomarkers of psychotic spectrum disorders in the early stages. (C) 2013 Elsevier Ltd. All rights reserved.