6-Benzylaminopurine delays senescence and enhances health-promoting compounds of harvested broccoli.

6-Benzylaminopurine delays senescence and enhances health-promoting compounds of harvested broccoli.
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DOI:
10.1021/jf2040884
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发表时间:
2012-01
影响因子:
6.1
通讯作者:
Feng Xu;Zhenfeng Yang;Xuehong Chen;P. Jin;Xiaoli Wang;Yonghua Zheng
Feng Xu;Zhenfeng Yang;Xuehong Chen;P. Jin;Xiaoli Wang;Yonghua Zheng
中科院分区:
农林科学1区
文献类型:
--
作者:
Feng Xu;Zhenfeng Yang;Xuehong Chen;P. Jin;Xiaoli Wang;Yonghua Zheng

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研究了6 - 苄氨基嘌呤(6 - BA)对西兰花小花颜色、抗氧化活性以及总酚、硫代葡萄糖苷和萝卜硫素含量的影响。结果表明,6 - BA处理显著抑制了L*值和丙二醛(MDA)含量的增加,并延缓了H值的降低。6 - BA处理通过调节叶绿素酶和镁脱螯合酶的活性降低了叶绿素的降解速率。与对照小花相比,经6 - BA处理的小花的1,1 - 二苯基 - 2 - 苦基肼基(DPPH)自由基清除活性以及超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)和过氧化氢酶(CAT)的活性增强,而过氧化物酶(POD)的活性则显著降低。经6 - BA处理后,西兰花小花中的总酚、硫代葡萄糖苷和萝卜硫素含量也大幅增加。这些结果表明,6 - BA能够保持西兰花的品质,延缓衰老,并提高其营养价值。
The effect of 6-benzylaminopurine (6-BA) on the color, antioxidant activity, and contents of total phenols, glucosinolate, and sulforaphane in broccoli florets was investigated. The results showed that 6-BA treatment markedly inhibited the increase of the L* value and malondialdehyde (MDA) content and retarded the decrease of the H value. 6-BA treatment reduced the rate of chlorophyll degradation by regulating the activities of chlorophyllase and Mg-dechelatase. When compared to control florets, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) were enhanced in florets treated with 6-BA, whereas the activity of peroxidase (POD) was significantly reduced. The contents of total phenols, glucosinolate, and sulforaphane in broccoli florets were also profoundly increased after treatment with 6-BA. These results indicated that 6-BA could maintain the quality, delay senescence, and improve the nutritional value of broccoli.