Immunotoxicity of F-53B, an alternative to PFOS, on zebrafish (Danio rerio) at different early life stages
Immunotoxicity of F-53B, an alternative to PFOS, on zebrafish (Danio rerio) at different early life stages
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F-53B（全氟辛烷磺酸替代品）对斑马鱼（斑马鱼）不同早期生命阶段的免疫毒性
DOI：
10.1016/j.scitotenv.2021.148165
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发表时间：
2021-06-08
影响因子：
9.8
通讯作者：
Tu, Wenqing
中科院分区：
文献类型：
--
作者：
Liu, Shuai;Lai, Hong;Tu, Wenqing
作为全氟辛烷磺酸(PFOS)的替代品，6：2氯化聚氟醚磺酸盐(F53B)近年来在我国市场上出现，并在水环境中经常被检测到，但其生态风险评估有限。斑马鱼胚胎和仔鱼分别暴露于F53B，96h的半数致死浓度(LC_(50))分别为15.1 mg/L和2.4 mg/L，表明胚胎对F53B的抗性高于仔鱼。幼虫体内的生物浓缩系数基本高于胚胎，F53B对幼虫生长的影响显著大于胚胎，说明F53B对幼虫的毒性可能更大。除了过量产生ROS和NO外，许多免疫相关基因在胚胎和幼虫中的表达都增加，但在幼虫中表达异常的基因数量多于胚胎。POD结果表明，F53B对幼虫的免疫毒性在分子水平上比对胚胎更敏感。通过探讨免疫调节对斑马鱼不同生命阶段的不利影响，揭示了F53B的生态风险，并表明斑马鱼幼体比胚胎更敏感。(C)2021年爱思唯尔B.V.保留所有权利。上标/下标可用
As an alternative to perfluorooctane sulfonate (PFOS), 6:2 chlorinated polyfluorinated ether sulfonate (F\\53B) has emerged in the Chinese market in recent years and has been frequently detected in the aquatic environment, but its ecological risk assessment is limited. In this study, zebrafish embryos and larvae were separately exposed to F\\53B, and their 96-h LC50 values were estimated to be 15.1 mg/L and 2.4 mg/L, respectively, suggesting that embryos were more resistant to F\\53B than larvae. The bioconcentration factor in larvae was basically higher than that of embryos, and the body growth of larvae was significantly affected by F\\53B rather than embryos, indicating that F\\53B may cause more severe toxicity to larvae. In addition to the excessive production of ROS and NO, the expression of many immune-related genes was increased in both embryos and larvae, but the number of dysregulated genes in larvae was more than that in embryos. Finally, the results of Point of Departure (PoD) indicated that the immunotoxicity of F\\53B was more sensitive to larvae than embryos at the molecular level. Our findings revealed the ecological risk of F\\53B by exploring the adverse effects of immunoregulation at different early life stages of zebrafish and indicated that the zebrafish larvae were more sensitive than embryos. (c) 2021 Elsevier B.V. All rights reserved.Superscript/Subscript Available