Age-dependent and gender-specific changes in mouse tissue iron by strain
Age-dependent and gender-specific changes in mouse tissue iron by strain
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DOI：
10.1016/j.exger.2009.06.006
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发表时间：
2009-09-01
影响因子：
3.9
通讯作者：
Dunaief, Joshua L.
中科院分区：
文献类型：
--
作者：
Hahn, Paul;Song, Ying;Dunaief, Joshua L.
铁是生命所必需的，但也是一种有效的促氧化剂，与年龄相关疾病的发病机制有关。我们试图确定铁水平是否随年龄和性别的变化，在各种组织从几个常用的研究小鼠品系。从年轻成年（2-6月龄）和老年（16-19月龄）C57 BL/6、DBA/2 J和BALB/c小鼠的雄性和雌性小鼠解剖脑、肝、心脏、视网膜和视网膜色素上皮（RPE）/脉络膜。通过基于发色团的分光光度法或通过原子吸收分光光度法对铁进行定量，以提高灵敏度。所有品系的老年组与年轻组相比，大脑、肝脏和心脏铁增加了30-70%，而视网膜和RPE/脉络膜铁具有可变的年龄相关变化。在BALB/c和DBA/2 J品系中观察到显著的性别差异。男性的大脑、RPE/脉络膜和视网膜铁含量是女性的2- 3倍，而女性的肝脏铁含量是男性的2- 3倍。在心脏铁方面没有观察到显著的性别差异。性别之间和品系之间变化的不同概况表明，激素和遗传影响铁调节与衰老。未来对小鼠体内铁水平的操作将测试铁在衰老和疾病中的作用，本文报道的数据对于指导此类操作至关重要。(C)2009 Elsevier Inc. All rights reserved.
Iron is necessary for life but also a potent pro-oxidant implicated in the pathogenesis of age-related diseases. We sought to determine if iron levels change with age and by sex in various tissues from several commonly studied mouse strains. Brain, liver, heart, retina, and retinal pigment epithelium (RPE)/choroid were dissected from male and female mice of young adult (2-6 month old) and aged (16-19 month old) C57BL/6, DBA/2J, and BALB/c mice. Iron was quantified through a chromagen-based spectrophotometric method or through atomic absorption spectrophotometry for increased sensitivity. Brain, liver, and heart iron increased by 30-70% in aged vs. young adult groups of all strains, while retina and RPE/choroid iron had variable age-related changes. Significant gender differences were observed in BALB/c and DBA/2J strains. Males had as much as 2- to 3-fold more brain, RPE/choroid, and retinal iron, while females had as much as 2- to 3-fold more liver iron. There was no significant gender difference observed in heart iron. The different profiles of change between gender and among strains suggest that hormones and genetics influence iron regulation with aging. Future manipulation of iron levels in mice will test the role of iron in aging and disease, and the data reported herein will be essential in directing such manipulations. (C) 2009 Elsevier Inc. All rights reserved.