In vitro characterization of an avian reovirus vaccine strain.
In vitro characterization of an avian reovirus vaccine strain.
复制标题
禽呼肠孤病毒疫苗株的体外表征。
DOI：
10.1016/0042-6822(83)90475-0
复制
发表时间：
1983
期刊：
影响因子：
3.7
通讯作者：
Schnitzer,TJ
中科院分区：
文献类型：
--
作者：
Gouvea,V;Huang,DD;Ramos,T;Schnitzer,TJ
In vitrostudies were performed to characterize the vaccine strain, designated P100, derived from the arthrogenic reovirus isolate, S1133, by vold adaptation. P100 appeared to be temperature sensitive, shown by a marked drop in titer and efficiency of plaquing after incubation at 41°. Studies indicated that genomic double-stranded RNA and protein synthesis were severely restricted at the elevated temperature. Differences in the growth behavior of P100 and S1133 at 37° were also noted. The vaccine strain seemed to be more cell associated than S1133. Three outer coat proteins of P100 grown at 37° displayed mobilities different from those of S1133 by PAGE. It is possible that alterations in these proteins may have some relationship to the growth characteristics observed for the P100 strain.
