Expression profiling of the whole Arabidopsis Shaggy-like kinase multigene family by real-time reverse transcriptase-polymerase chain reaction
Expression profiling of the whole Arabidopsis Shaggy-like kinase multigene family by real-time reverse transcriptase-polymerase chain reaction
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DOI：
10.1104/pp.009175
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发表时间：
2002-10-01
期刊：
影响因子：
7.4
通讯作者：
Kreis, M
中科院分区：
文献类型：
--
作者：
Charrier, B;Champion, A;Kreis, M
最近发表的拟南芥基因组的完整序列，使我们能够确定和表征的最后两个成员的SHAGGY样激酶（AtSK）基因家族。因此，研究拟南芥中AtSK家族的整体时空组织结构已成为了解每个SHAGGY样激酶在植物发育过程中的作用的一个可实现和必要的目标。使用实时定量逆转录-聚合酶链反应技术对该家族10个成员的转录水平进行了分析。分析了不同生长条件下几个器官中的转录水平。为了校准所获得的结果，使用了许多其他基因，例如编码两个MAP 3 Kes和两个MAP 4Kalphas的那些基因，以及胁迫响应标记RD 29 A; Rubisco光合酶AtS 1A的小亚基; MEDEA染色质重塑因子;以及SCARECROW、ASYMMETRIC LEAVES 1和SUPERMAN转录因子，它们都参与植物发育的关键步骤。我们的数据的分析表明，8的10个基因的AtSK家族显示在器官水平的假组成型表达模式。相反，AtSK 13响应于渗透压变化和盐水处理，而AtSK 31是花特异性的，并响应于渗透压变化和黑暗。
The recent publication of the complete sequence of the Arabidopsis genome allowed us to identify and characterize the last two members of the SHAGGY-like kinase (AtSK) gene family. As a result, the study of the overall spatio-temporal organization of the whole AtSK family in Arabidopsis has become an achievable and necessary aim to understand the role of each SHAGGY-like kinase during plant development. An analysis of the transcript level of the 10 members of the family has been performed using the technique of real-time quantitative reverse transcriptase-polymerase chain reaction. Transcript levels in several organs, under different growth conditions, were analyzed. To calibrate the results obtained, a number of other genes, such as those coding for the two MAP3Kes and the two MAP4Kalphas, as well as the stress response marker RD29A; the small subunit of the Rubisco photosynthetic enzyme AtS1A; the MEDEA chromatin remodeling factor; and the SCARECROW, ASYMMETRIC LEAVES 1, and SUPERMAN transcription factors all involved in key steps of plant development were used. The analysis of our data revealed that eight of the 10 genes of the AtSK family displayed a pseudo-constitutive expression pattern at the organ level. Conversely, AtSK13 responded to osmotic changes and saline treatment, whereas AtSK31 was flower specific and responded to osmotic changes and darkness.