Microarray expression profile analysis of mRNAs and long non-coding RNAs in pulmonary tuberculosis with different traditional Chinese medicine syndromes.
Microarray expression profile analysis of mRNAs and long non-coding RNAs in pulmonary tuberculosis with different traditional Chinese medicine syndromes.
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肺结核不同中医证型中mRNA和长非编码RNA的微阵列表达谱分析
DOI：
10.1186/s12906-016-1436-y
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发表时间：
2016-11-17
影响因子：
--
通讯作者：
Li JC
中科院分区：
文献类型：
--
作者：
Jiang TT;Wei LL;Shi LY;Chen ZL;Wang C;Liu CM;Li ZJ;Li JC
联合化疗与西方抗结核药物（TB）是结核病治疗的主流。根据结核病患者的中医证候，采用清热解毒或滋阴降火的中草药治疗结核病。本研究分析了长链非编码rna （lncRNAs）和mrna在不同中医证型结核患者中的表达谱。结核患者分为肺阴虚证（PYD）、阴虚火上亢证（HFYD）和气阴虚证（DQY）。从44名结核病患者和健康对照中提取总RNA，并与包含30586个lncRNA和26109个mrna探针的人lncRNA微阵列进行杂交。进行了生物信息学分析，包括基因本体（GO）和途径。并对相关临床资料进行分析。PYD患者（634个mrna和566个lncRNAs）、HFYD患者（47个mrna和55个lncRNAs）和DQY患者（63个mrna和60个lncRNAs）中鉴定出差异表达的mrna和lncRNAs （fold change bbb2, P < 0.05）。富集最多的通路是hippo信号通路（P = 0.000164）和蛋白质消化吸收通路（P = 5.89017E-05）。临床分析显示，TB患者血脂指标异常，DQY患者甘油三酯浓度显著升高（P = 0.0252）。我们的研究首次获得了lncRNAs和mrna的微阵列表达谱，并分析了PYD、HFYD和DQY结核病患者的通路富集情况。我们对lncRNAs和mrna表达谱的分析可能为探索结核中医证候的生物学本质提供了一种新的方法。本文的在线版本（doi:10.1186/s12906-016-1436-y）包含补充材料，可供授权用户使用。
Combination chemotherapy with Western anti-tuberculosis (TB) drugs is the mainstay of TB treatment. Chinese herbal medicines with either heat clearing and detoxifying effects or nourishing Yin and reducing fire effects have been used to treat TB based on the Traditional Chinese Medicine (TCM) syndromes of TB patients. This study analyzed the expression profiles of long non-coding RNAs (lncRNAs) and mRNAs in TB patients with different TCM syndromes. TB patients were classified as pulmonary Yin deficiency (PYD) syndrome, hyperactivity of fire due to Yin deficiency (HFYD) syndrome, and deficiency of Qi and Yin (DQY) syndrome. Total RNA from 44 TB patients and healthy controls was extracted and hybridized with a human lncRNA microarray containing 30586 lncRNAs and 26109 mRNAs probes. Bioinformatics analyses, including gene ontology (GO) and pathways, were performed. Related clinical data were also analyzed. Differentially expressed mRNAs and lncRNAs were identified (fold change >2, and P < 0.05) in PYD (634 mRNAs and 566 lncRNAs), HFYD (47 mRNAs and 55 lncRNAs), and DQY (63 mRNAs and 60 lncRNAs) patients. The most enriched pathways were the hippo signaling pathway (P = 0.000164) and the protein digestion and absorption pathway (P = 5.89017E-05). Clinical analyses revealed that the lipid indexes of TB patients were abnormal and that the triglyceride concentration was significantly higher in DQY patients (P = 0.0252). Our study is the first to acquire the microarray expression profiles of lncRNAs and mRNAs and analyze pathway enrichment in PYD, HFYD, and DQY patients with TB. Our analyses of the expression profiles of lncRNAs and mRNAs may represent a novel method to explore the biological essence of TCM syndromes of TB. The online version of this article (doi:10.1186/s12906-016-1436-y) contains supplementary material, which is available to authorized users.
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影响因子：
14.8
作者：
Arora, Pooja;Goyal, Aneesh;Natarajan, Vivek T.;Rajakumara, Eerappa;Verma, Priyanka;Gupta, Radhika;Yousuf, Malikmohamed;Trivedi, Omita A.;Mohanty, Debasisa;Tyagi, Anil;Sankaranarayanan, Rajan;Gokhale, Rajesh S.
通讯作者：
Gokhale, Rajesh S.
影响因子：
3.5
作者：
Muhammad E;Reish O;Ohno Y;Scheetz T;Deluca A;Searby C;Regev M;Benyamini L;Fellig Y;Kihara A;Sheffield VC;Parvari R
通讯作者：
Parvari R
影响因子：
--
作者：
EVANS, DAP;MANLEY, KA;MCKUSICK, VA
通讯作者：
MCKUSICK, VA
影响因子：
82.9
作者：
Muñoz-Elías, EJ;McKinney, JD
通讯作者：
McKinney, JD
影响因子：
4.8
作者：
Hunt, Mary C.;Rautanen, Anna;Alexson, Stefan E. H.
通讯作者：
Alexson, Stefan E. H.