Transgenic Wheat, Barley and Oats
Transgenic Wheat, Barley and Oats
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转基因小麦、大麦和燕麦
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
P. Shewry
中科院分区：
文献类型：
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作者：
H. Jones;P. Shewry
自20世纪80年代初利用模式植物物种的植物转化技术首次发展以来，已经有一个植物科学研究机构致力于将这些技术应用于作物植物的转化。对于一些作物物种来说，进展相对较快，但对于其他作物群体，如小谷物，它们不容易适应体外培养，也不是农杆菌的天然宿主，花了近20年的时间才开发出可靠和强大的转化方法。在本书的以下章节中，介绍了小粒谷物的转化过程，以及基因和蛋白质表达的方法和转基因植物的特性。在这一导论章中，我们试图将后面的章节置于上下文中，概述小粒谷物转化技术的发展，讨论这些技术的一些优点和缺点以及仍然存在的局限性。
Ever since the first developments in plant transformation technology using model plant species in the early 1980s, there has been a body of plant science research devoted to adapting these techniques to the transformation of crop plants. For some crop species progress was relatively rapid, but in other crop groups such as the small grain cereals, which were not readily amenable to culture in vitro and were not natural hosts to Agrobacterium , it has taken nearly two decades to develop reliable and robust transformation methods. In the following chapters of this book, transformation procedures for small grain cereals are presented, together with methods for gene and protein expression and the characterization of transgenic plants. In this introductory chapter we try to put these later chapters into context, giving an overview of the development of transformation technology for small grain cereals, discussing some of the pros and cons of the techniques and what limitations still exist.