Effects of the sliaa9 Mutation on Shoot Elongation Growth of Tomato Cultivars.
Effects of the sliaa9 Mutation on Shoot Elongation Growth of Tomato Cultivars.
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SLIAA9突变对番茄品种芽伸长生长的影响。
DOI：
10.3389/fpls.2021.627832
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发表时间：
2021
影响因子：
5.6
通讯作者：
Osakabe Y
中科院分区：
文献类型：
--
作者：
Abe-Hara C;Yamada K;Wada N;Ueta R;Hashimoto R;Osakabe K;Osakabe Y
番茄吲哚-3-乙酸9（SlIAA 9）是生长素信号转导中的转录阻遏物，并且SlIAA 9敲除的番茄植物在没有受精的情况下发育单性结实果实。我们使用CRISPR-Cas9在几个商业番茄栽培品种（Moneymaker、格兰德河和Ailsa克雷格）中产生了具有单性结实的sliaa 9突变体，并且通过自花授粉分离了T1代中的空分离株系，这通过PCR和Southern印迹分析证实。然后，我们估计芽生长表型的突变体植物在不同的光（低和正常）条件下。Moneymaker和格兰德河的sliaa 9突变体在弱光条件下的茎长小于野生型，而Ailsa克雷格突变体在弱光条件下的茎长与野生型无明显差异。此外，格兰德河幼苗下胚轴生长表现出避荫反应，在弱光条件下胚轴长度增加，而Ailsa克雷格的sliaa 9突变体幼苗在该表型中表现出增强的反应。果实产量和生长速率在sliaa 9突变体番茄品种之间是相似的。这些结果表明，AUX/IAA 9和光条件的相互作用在延长生长的控制机制参与商业番茄品种之间的差异。
Tomato INDOLE-3-ACETIC ACID9 (SlIAA9) is a transcriptional repressor in auxin signal transduction, and SlIAA9 knockout tomato plants develop parthenocarpic fruits without fertilization. We generated sliaa9 mutants with parthenocarpy in several commercial tomato cultivars (Moneymaker, Rio Grande, and Ailsa Craig) using CRISPR-Cas9, and null-segregant lines in the T1 generation were isolated by self-pollination, which was confirmed by PCR and Southern blot analysis. We then estimated shoot growth phenotypes of the mutant plants under different light (low and normal) conditions. The shoot length of sliaa9 plants in Moneymaker and Rio Grande was smaller than those of wild-type cultivars in low light conditions, whereas there was not clear difference between the mutant of Ailsa Craig and the wild-type under both light conditions. Furthermore, young seedlings in Rio Grande exhibited shade avoidance response in hypocotyl growth, in which the hypocotyl lengths were increased in low light conditions, and sliaa9 mutant seedlings of Ailsa Craig exhibited enhanced responses in this phenotype. Fruit production and growth rates were similar among the sliaa9 mutant tomato cultivars. These results suggest that control mechanisms involved in the interaction of AUX/IAA9 and lights condition in elongation growth differ among commercial tomato cultivars.
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影响因子：
5.6
作者：
Hashimoto R;Ueta R;Abe C;Osakabe Y;Osakabe K
通讯作者：
Osakabe K
影响因子：
3.5
作者：
Motohashi K
通讯作者：
Motohashi K
影响因子：
7.4
作者：
Colón-Carmona, A;Chen, DL;Abel, S
通讯作者：
Abel, S
影响因子：
4.6
作者：
Koenig, Daniel;Bayer, Emmanuelle;Sinha, Neelima
通讯作者：
Sinha, Neelima
影响因子：
7.4
作者：
Ariel Trupkin, Santiago;Legris, Martina;Jose Casal, Jorge
通讯作者：
Jose Casal, Jorge