Mash1 is required for glomus cell formation in the mouse carotid body.
Mash1 is required for glomus cell formation in the mouse carotid body.
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DOI：
10.1016/j.ydbio.2005.04.004
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发表时间：
2005-07
影响因子：
2.7
通讯作者：
Y. Kameda
中科院分区：
文献类型：
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作者：
Y. Kameda
颈动脉体由化学感受球细胞、支持细胞和神经末梢组成。小鼠颈动脉体位于颈动脉分叉处，始终与交感干的颈上神经节相连。血管球细胞和交感神经元对 TuJ1、PGP9.5、酪氨酸羟化酶 (TH) 和神经肽 Y (NPY) 标记物具有免疫反应性。血管球细胞也对血清素 (5-HT) 产生免疫反应。 Mash1（果蝇-盾片复合体的小鼠同源物）的靶向突变导致交感神经节的消除。在Mash1缺失突变小鼠中，颈动脉体原基在胚胎日（E）13.0时在第三弓动脉壁中正常形成并继续发育，尽管颈上神经节完全不存在。然而，突变颈动脉体中的细胞在整个发育过程中没有显示 TuJ1、PGP 9.5、TH、NPY 和 5-HT 标记。电子显微镜也证实了不存在血管球细胞。新生无效突变体的颈动脉体由间充质样细胞和神经纤维组成。在胎儿发育过程中，许多对 S-100 蛋白（一种支持细胞标记物）具有免疫反应的细胞出现在突变的颈动脉体中。因此，Mash1 基因是血管球细胞发生所必需的，但对于支持细胞而言则不然。
The carotid body consists of chemoreceptive glomus cells, sustentacular cells and nerve endings. The murine carotid body, located at the carotid bifurcation, is always joined to the superior cervical ganglion of the sympathetic trunk. Glomus cells and sympathetic neurons are immunoreactive for the TuJ1, PGP9.5, tyrosine hydroxylase (TH) and neuropeptide Y (NPY) markers. Glomus cells are also immunoreactive for serotonin (5-HT). A targeted mutation of Mash1, a mouse homolog of the Drosophila achaete–scute complex, results in the elimination of sympathetic ganglia. In Mash1 null mutant mice, the carotid body primordium forms normally in the wall of the third arch artery at embryonic day (E) 13.0 and continues to develop, although the superior cervical ganglion is completely absent. However, no cells in the mutant carotid body display the TuJ1, PGP 9.5, TH, NPY and 5-HT markers throughout development. The absence of glomus cells was also confirmed by electron microscopy. The carotid body of newborn null mutants is composed of mesenchymal-like cells and nerve fibers. Many cells immunoreactive for the S-100 protein, a sustentacular cell marker, appear in the mutant carotid body during fetal development. The Mash1 gene is thus required for the genesis of glomus cells but not for sustentacular cells.