Rapid detection protocol for filoviruses
Rapid detection protocol for filoviruses
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DOI：
10.1016/j.jcv.2003.09.004
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发表时间：
2004-05-01
影响因子：
8.8
通讯作者：
Hufert, FT
中科院分区：
文献类型：
--
作者：
Weidmann, M;M端hlberger, E;Hufert, FT
背景：近年来，丝状病毒病暴发的发病率呈上升趋势。虽然诊断学的发展取得了进展，但现场试验很少。除受感染患者的家庭成员外，卫生保健工作者在疫情暴发的初始阶段受到感染的风险很高。RT-PCR已被证明有助于控制疫情。目的：建立埃博拉-扎伊尔病毒（EBOV-Z）、埃博拉-苏丹病毒（EBOV-S）和马尔堡病毒（MBGV）的Taqman-RT-PCR检测方法。研究设计：在便携式Smartcycler TD上开发并建立了用于检测这些病毒的定量taqman - rt - pcr。结果与结论：三种检测方法均具有较高的敏感性和特异性。检测系统的移动性可能有助于控制未来的疫情。(C) 2003 Elsevier B.V.版权所有
Background: The incidence of filovirus disease outbreaks has been increasing in recent years. Although there have been advances in the developments of diagnostics, field tests are rare. Apart from family members of infected patients, health care workers are at high risk of being infected during the initial phase of an outbreak. RT-PCR has been shown to be helpful in containing outbreaks.Objectives: To develop Taqman-RT-PCR for the detection of Ebola-Zaire virus (EBOV-Z), Ebola-Sudan virus (EBOV-S) and Marburg virus (MBGV).Study desgin: Quantitative Taqman-RT-PCRs for the detection of these viruses were developed and established on a portable Smartcycler TD.Results and conclusions: All three assays were highly sensitive and specific. The mobility of the assay system may help to contain future outbreaks. (C) 2003 Elsevier B.V. All rights reserved.