Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway components.
Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway components.
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DOI：
10.1242/bio.018440
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发表时间：
2016-06-15
期刊：
影响因子：
2.4
通讯作者：
Cobb JA
中科院分区：
文献类型：
--
作者：
Bustard DE;Ball LG;Cobb JA
酿酒酵母中的Smc 5/6复合物包含六个必需的非Smc元件Nse 1 -6。除了Nse 2（也称为Mms 21），这是一种E3小泛素样修饰物（SUMO）连接酶，很少有人了解这些组分的作用或它们对Smc 5/6功能的贡献。我们对Nse 5的表征建立了Smc 5/6复合物与SUMO途径因子之间先前未识别的关系。Nse 5与E2缀合酶Ubc 9物理结合，其中接触通过与SUMO的非共价相互作用而稳定。SUMO还介导Nse 5与两个皮亚斯家族E3 SUMO连接酶Siz 1和Siz 2之间的相互作用。携带nse 5-ts 1等位基因或缺乏SIZ 1或SIZ 2的细胞在MMS处理后表现出Smc 5类小泛素化的减少，并证明在DNA损伤存在下SUMO介导的事件的功能冗余。总的来说，鉴于Nse 5和SUMO通路的组分之间的广泛联系，我们推测Smc 5/6复合物的一个功能可能是作为支架中心，使sumoylation事件在芽殖酵母。摘要：在这里，我们描述了Smc 5/6复合物的一个成分Nse 5的特征。Nse 5与皮亚斯E3 SUMO连接酶Siz 1和Siz 2相互作用，并调节Smc 5类小泛素化。
The Smc5/6 complex in Saccharomyces cerevisiae contains six essential non-Smc elements, Nse1-6. With the exception of Nse2 (also known as Mms21), which is an E3 small ubiquitin-like modifier (SUMO) ligase, very little is understood about the role of these components or their contribution to Smc5/6 functionality. Our characterization of Nse5 establishes a previously unidentified relationship between the Smc5/6 complex and factors of the SUMO pathway. Nse5 physically associates with the E2 conjugating enzyme, Ubc9, where contacts are stabilized by non-covalent interactions with SUMO. SUMO also mediates the interactions between Nse5 and the two PIAS family E3 SUMO ligases, Siz1 and Siz2. Cells carrying the nse5-ts1 allele or lacking either SIZ1 or SIZ2 exhibit a reduction in Smc5 sumoylation upon MMS treatment and demonstrate functional redundancy for SUMO mediated events in the presence of DNA damage. Overall, given the extensive connection between Nse5 and components of the SUMO pathway, we speculate that one function of the Smc5/6 complex might be as a scaffold center to enable sumoylation events in budding yeast. Summary: Here we characterize Nse5, a component of the Smc5/6 complex. Nse5 interacts with the PIAS E3 SUMO ligases, Siz1 and Siz2, and regulates Smc5 sumoylation.