Analysis of the Relationship Between the Degree of Dysbiosis in Gut Microbiota and Prognosis at Different Stages of Primary Hepatocellular Carcinoma
Analysis of the Relationship Between the Degree of Dysbiosis in Gut Microbiota and Prognosis at Different Stages of Primary Hepatocellular Carcinoma
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DOI：
10.3389/fmicb.2019.01458
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发表时间：
2019-06-25
影响因子：
5.2
通讯作者：
Gao, Yi
中科院分区：
文献类型：
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作者：
Ni, Jiajia;Huang, Rong;Gao, Yi
肠道微生物群失调与原发性肝细胞癌（HCC）密切相关。最近的研究通过分析肠道微生物群失调来评估原发性肝癌的早期诊断。然而，微生态失调的程度和原发性肝癌的预后之间的关系仍然不清楚。由于原发性肝癌伴随着生态失调，而生态失调通常会增加内毒素和其他有害细菌物质的循环浓度，从而进一步增加肝损伤，因此我们假设与原发性肝癌相关的生态失调水平随着癌症进展而增加。为了验证这一假设，我们引入了一个更综合的指标，称为生态失调程度（D-dys）;我们通过对16 S rRNA基因扩增子的高通量测序，研究了肠道微生物群的D-dys与原发性肝癌的发展。我们的研究结果表明，与健康个体相比，原发性肝癌患者的粪便微生物群中的促炎细菌增加。原发性肝癌患者D-dys明显高于健康对照组。此外，有一个趋势，为D-dys增加与原发性肝癌的发展，虽然没有检测到原发性肝癌的不同阶段之间的显着差异。我们的研究结果为在原发性肝癌治疗期间使用肠道微生物群分析提供了重要见解。
Gut microbiota dysbiosis is closely associated with primary hepatocellular carcinoma (HCC). Recent studies have evaluated the early diagnosis of primary HCC through analysis of gut microbiota dysbiosis. However, the relationship between the degree of dysbiosis and the prognosis of primary HCC remains unclear. Because primary HCC is accompanied by dysbiosis and dysbiosis usually increases the circulatory concentrations of endotoxin and other harmful bacterial substances, which further increases liver damage, we hypothesized that level of dysbiosis associated with primary HCC increases with the stage of cancer progression. To test this hypothesis, we introduced a more integrated index referred to as the degree of dysbiosis (D-dys); and we investigated D-dys of the gut microbiota with the development of primary HCC through high-throughput sequencing of 16S rRNA gene amplicons. Our results showed that compared with healthy individuals, patients with primary HCC showed increased pro-inflammatory bacteria in their fecal microbiota. The D-dys increased significantly in patients with primary HCC compared with that in healthy controls. Moreover, there was a tendency for the D-dys to increase with the development of primary HCC, although no significant difference was detected between different stages of primary HCC. Our findings provide important insights into the use of gut microbiota analysis during the treatment of primary HCC.