Inter-ocular and inter-session reliability of the electroretinogram photopic negative response (PhNR) in non-human primates
Inter-ocular and inter-session reliability of the electroretinogram photopic negative response (PhNR) in non-human primates
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DOI：
10.1016/j.exer.2003.09.013
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发表时间：
2004-01-01
影响因子：
3.4
通讯作者：
Cioffi, GA
中科院分区：
文献类型：
--
作者：
Fortune, B;Bui, BV;Cioffi, GA
目的。目的：评估一组正常非人类灵长类动物明视视网膜电信号(ERG)参数的眼间和节间可靠性。在一组正常麻醉的成年恒河猴(恒河猴，29只)中评估了明视ERG的眼间差异；3个月后对23只动物的23只眼的会话间可靠性进行了评估。使用Burian-Allen接触镜电极采集信号，对侧角膜作为参照物。使用红色Ganzfeld闪光(-0.5-0.67log明视cdm(-2))在抑制蓝色背景的棒(30暗光cdm(-2))上激发光敏ERG。通过计算每个参数的95%的一致性限(LOA)和变异系数(COV)，建立a波、b波、明视负反应(PhNR)和振荡电位(OP)的幅度及其隐含时间的测量可靠性。OP波和a波的幅度随强度增加而增加，最高可达0.67log明视cd.sm(-2)，符合典型的饱和函数，而b波和PhnR波的幅度均下降到0.42log明视cd.sm(-2)以上。节间变异性大于眼球间变异性。PhNR波幅(10~20%)与其他明视ERG成分(a波：12~17%，b波：12~17%，OPs：13~19%)相似。各组分的会话间LOA也是相似的，但平均而言，对中等强度(0.0-0.42log明视cd.ms(-2))的反应最小。在非人类灵长类动物中，明视ERG a波、b波、OPs和PhNR节间测量的95%LOA都是相似的。对于中等明亮的刺激强度，最好使用PhNR波幅来测量视网膜内损伤。对于更亮的闪光灯，B波和PhNR的幅度更小，变化更大。PhNR：B波波幅的比值具有较小的变异性，因此可用于检测选择性PhNR的丢失。(C)2004爱思唯尔有限公司。保留所有权利。
Purpose. To assess the inter-ocular and inter-session reliability for a range of parameters derived from the photopic electroretinogram (ERG) in a group of normal non-human primates.Methods. Inter-ocular differences for photopic ERGs were assessed in a group of normal anesthetized adult rhesus monkeys (Macaca mulatta, it = 29); inter-session reliability was assessed for 23 eyes of 23 animals tested 3 months later. Signals were acquired using Burian-Allen contact lens electrodes, whereby the contralateral cornea served as a reference. Photopic ERGs were elicited using red Ganzfeld flashes (- 0.5-0.67 log photopic cd.s m(-2)) on a rod suppressing blue-background (30 scotopic cd m(-2)). Measurement reliability was established for a-wave, b-wave, photopic negative response (PhNR) and oscillatory potential (OP) amplitudes, as well as for their implicit times, by calculation of the 95% limits-of-agreement (LOA) and the coefficient-of-variation (COV) for each parameter.Results. OP and a-wave amplitudes increased with intensity up to 0.67 log photopic cd.s m(-2), following a typical saturating function, whereas b-wave and PhNR amplitudes both declined above 0.42 log photopic cd.s m(-2). Inter-session variability was greater than inter-ocular variability. The inter-session COVs for PhNR amplitude (10-20%) were similar to the other photopic ERG components (a-wave: 12-17%, b-wave: 12-17%, OPs: 13-19%). Inter-session LOAs were also similar across components, but on average, were smallest for responses to moderate intensities (0.0-0.42 log photopic cd.s m(-2)).Conclusion. In non-human primates, the 95% LOA for inter-session measurements of the photopic ERG a-wave, b-wave, OPs and PhNR are all similar. Inner-retinal damage may best be measured using the PhNR amplitude for moderately bright stimulus intensities. B-wave and PhNR amplitudes for brighter flashes are smaller and more variable. The ratio of PhNR:b-wave amplitudes manifests smaller variability and may therefore be useful for detection of selective PhNR loss. (C) 2004 Elsevier Ltd. All rights reserved.