Thermal stable characteristics of acid- and pepsin-soluble collagens from the carapace tissue of Chinese soft-shelled turtle (Pelodiscus sinensis)
Thermal stable characteristics of acid- and pepsin-soluble collagens from the carapace tissue of Chinese soft-shelled turtle (Pelodiscus sinensis)
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中华鳖甲壳组织中酸溶性和胃蛋白酶溶性胶原蛋白的热稳定性特性
DOI：
10.1016/j.tice.2020.101424
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发表时间：
2020-12-01
期刊：
影响因子：
2.6
通讯作者：
Qian, Guo-Ying
中科院分区：
文献类型：
--
作者：
Li, Caiyan;Song, Wei;Qian, Guo-Ying
中国软壳龟（Pelodiscus sinensis）的甲壳被用作传统中药。本研究从甲壳中分离并鉴定了酸溶性胶原蛋白（ASC）和胃蛋白酶溶性胶原蛋白（PSC），以筛选新型胶原材料。以甘氨酸为主要氨基酸，亚胺酸含量高的ASC和PSC的产率分别为1.0%和2.8%。两种胶原蛋白的最大紫外吸收峰均为220 nm。SDS-PAGE显示两种胶原结构相似，属于I型胶原。胶原蛋白的相对粘度随着温度的升高而降低。胶原蛋白在pH为8时溶解度最小，盐浓度为3%时溶解度最大。PSC的变性温度（Td）高于ASC，而熔融温度（Td）低于ASC。扫描电镜显示ASC和PSC均呈海绵状微观结构，有纤维状孔隙。结果表明，甲壳胶原具有较高的热稳定性，在医药、食品和生物材料等领域具有替代哺乳动物胶原蛋白的潜力。但其生物学或药理活性有待进一步研究。
The carapace from the Chinese soft-shelled turtle (Pelodiscus sinensis) is used as a traditional Chinese medicine. Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from turtle carapace were isolated and characterized to screen novel collagen material in this study. Yields of 1.0% and 2.8% were obtained for ASC and PSC which contained glycine as the major amino acid and had high imino acid content. Both collagens had maximum ultraviolet absorption peaks of 220 nm. SDS-PAGE revealed that the structure of both collagens was similar, belonging to type I collagen. Relative viscosities of collagens were decreased as the temperature increased. Collagens showed minimum solubility at pH 8 and maximum solubility at a salt concentration of 3%. The denaturation temperature (Td) of PSC was higher whereas the melting temperature was lower than that of ASC. Both ASC and PSC appeared to be spongy like microstructure with fibrillar pores shown by scanning electron microscopy. The results suggest that collagens isolated from turtle carapace has high thermal stability with potential uses as new substitute for mammalian collagen in medicinal, food or biomaterial fields. However, their biological or pharmacological activities are needed to be further studied.