Proteome and phosphoproteome differential expression under salinity stress in rice (Oryza sativa) roots
Proteome and phosphoproteome differential expression under salinity stress in rice (Oryza sativa) roots
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DOI：
10.1021/pr060678z
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发表时间：
2007-01-01
影响因子：
4.4
通讯作者：
Peng, Zhaohua
中科院分区：
文献类型：
--
作者：
Chitteti, Brahmananda Reddy;Peng, Zhaohua
盐胁迫是限制世界范围内农业生产的主要非生物胁迫。水稻是单子叶植物（包括谷类作物）的模式植物。研究表明，蛋白质磷酸化在植物盐胁迫反应中起着关键作用。然而，水稻磷酸化蛋白质组，特别是在盐胁迫下，还没有得到很好的研究。本文利用Pro-Q Diamond磷蛋白染色法研究了盐胁迫下水稻磷蛋白质组的差异表达。17个差异上调和11个差异下调推定磷蛋白已被确定。进一步的分析表明，17个上调的蛋白质中的10个可能是在翻译后水平而不是蛋白质浓度上调。同时，我们还利用SYPRO Ruby染色鉴定了31个盐胁迫差异表达蛋白。虽然其中8个是已知的盐胁迫反应蛋白，但大多数尚未在文献中报道。我们的研究为植物对盐胁迫的响应提供了有价值的新见解。关键词：磷酸蛋白质组中心点水稻中心点盐胁迫中心点差异调节
Salinity stress is a major abiotic stress that limits agriculture productivity worldwide. Rice is a model plant of monocotyledons, including cereal crops. Studies have suggested a critical role of protein phosphorylation in salt stress response in plants. However, the phosphoproteome in rice, particularly under salinity stress, has not been well studied. Here, we use Pro-Q Diamond Phosphoprotein Stain to study rice phosphoproteome differential expression under salt stress. Seventeen differentially upregulated and 11 differentially downregulated putative phosphoproteins have been identified. Further analyses indicate that 10 of the 17 upregulated proteins are probably upregulated at post-translational level instead of the protein concentration. Meanwhile, we have identified 31 salt stress differentially regulated proteins using SYPRO Ruby stain. While eight of them are known salt stress response proteins, the majority has not been reported in the literature. Our studies have provided valuable new insight into plant response to salinity stress.Keywords: phosphoproteome center dot rice center dot salt stress center dot differential regulation