Whole Mitochondrial Genome Sequencing and Re-Examination of a Cytoplasmic Male Sterility-Associated Gene in Boro-Taichung-Type Cytoplasmic Male Sterile Rice.
Whole Mitochondrial Genome Sequencing and Re-Examination of a Cytoplasmic Male Sterility-Associated Gene in Boro-Taichung-Type Cytoplasmic Male Sterile Rice.
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DOI：
10.1371/journal.pone.0159379
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Toriyama K
中科院分区：
文献类型：
--
作者：
Kazama T;Toriyama K
亚种之间的核基因组替换可以通过核基因组和线粒体基因组之间的不相容性导致细胞质雄性不育（CMS）。通过对水稻亚种（Oryza sativa subsp.）Indica栽培品种Chinsurah Boro II与Oryza sativa subsp.粳稻台中65在BT型不育水稻中，线粒体基因orf79与雄性不育相关。一个完整的序列的博罗型线粒体基因组负责BT型CMS尚未确定的日期。在这里，我们使用焦磷酸测序来构建Boro型线粒体基因组。连续的序列被组装成五个环状DNA分子，其中四个可以连接成一个环。两个产生的亚基因组环无法形成可靠的主环，因为它们之间的重组几乎没有检测到。我们还发现atp6的两个位点的DNA分子丰度不等。这些结果表明，在博罗型线粒体基因组中存在多分裂的DNA分子。研究了Boro型拟南芥特异性orfs的表达模式，这些orfs在标准粳稻品种日本晴的线粒体中没有发现。在含有orf79的RNA中观察到育性恢复子1（RF1）依赖的RNA加工，但在其他Boro型线粒体特异性orf中未检测到，支持orf79是Boro型线粒体中独特的CMS相关基因的结论。
Nuclear genome substitutions between subspecies can lead to cytoplasmic male sterility (CMS) through incompatibility between nuclear and mitochondrial genomes. Boro-Taichung (BT)-type CMS rice was obtained by substituting the nuclear genome of Oryza sativa subsp. indica cultivar Chinsurah Boro II with that of Oryza sativa subsp. japonica cultivar Taichung 65. In BT-type CMS rice, the mitochondrial gene orf79 is associated with male sterility. A complete sequence of the Boro-type mitochondrial genome responsible for BT-type CMS has not been determined to date. Here, we used pyrosequencing to construct the Boro-type mitochondrial genome. The contiguous sequences were assembled into five circular DNA molecules, four of which could be connected into a single circle. The two resulting subgenomic circles were unable to form a reliable master circle, as recombination between them was scarcely detected. We also found an unequal abundance of DNA molecules for the two loci of atp6. These results indicate the presence of multi-partite DNA molecules in the Boro-type mitochondrial genome. Expression patterns were investigated for Boro-type mitochondria-specific orfs, which were not found in the mitochondria from the standard japonica cultivar Nipponbare. Restorer of fertility 1 (RF1)-dependent RNA processing has been observed in orf79-containing RNA but was not detected in other Boro-type mitochondria-specific orfs, supporting the conclusion that orf79 is a unique CMS-associated gene in Boro-type mitochondria.