Capacity of human beta-defensin expression in gene-transduced and cytokine-induced cells.
Capacity of human beta-defensin expression in gene-transduced and cytokine-induced cells.
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基因转导和细胞因子诱导的细胞中人β-防御素表达的能力。
DOI：
10.1016/j.bbrc.2005.11.020
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发表时间：
2006
影响因子：
3.1
通讯作者：
Huang,GeorgeT-J
中科院分区：
文献类型：
--
作者：
Yin,Chunyi;Dang,HoaN;Zhang,Hai-Bo;Gazor,Farzad;Kim,Daniel;Sorensen,OleE;Huang,GeorgeT-J
本研究的目的是确定用人β-防御素（HBD）转导的细胞表达抗微生物肽的能力，因为有效的抗微生物活性需要足够的表达水平。携带HBD的逆转录病毒载体pBabeNeo和慢病毒载体SIN 18 cPPTRhMLV（SIN 18）用于转染不表达HBD的细胞，如成纤维细胞或产生HBD的口腔上皮细胞。我们发现，HBD-3基因转移到成纤维细胞是可能的，而不是通过逆转录病毒，但通过直接载体转染。SIN 18具有高的转导效率（80.9-99.9%），转导的细胞表达的HBD-2的量高于pBabeNeo。慢病毒转导后，原代人牙龈上皮细胞（HGECs）表达的HBD-2量高于原代成纤维细胞。此外，当用IL-1或TNFα刺激时，转导的HGECs细胞的HBD-2分泌进一步增加。我们的数据表明，虽然HBD-2的表达是有限的原代成纤维细胞，其在HGECs的表达可以通过基因转导加细胞因子诱导最大化。
The purpose of this study was to determine the capacity of cells transduced with human β-defensins (HBDs) to express antimicrobial peptides, since sufficient expression level is required for effective antimicrobial activity. Retroviral vector pBabeNeo and lentiviral vector SIN18cPPTRhMLV (SIN18) carrying HBDs were utilized to transduce non-HBD-expressing cells such as fibroblasts or HBD-producing oral epithelial cells. We found that HBD-3 gene transfer to fibroblasts was possible not via retrovirus but by direct vector transfection. SIN18 had high transduction efficiencies (80.9–99.9%) and transduced cells expressed higher amounts of HBD-2 than those by pBabeNeo. Primary human gingival epithelial cells (HGECs) expressed greater amounts of HBD-2 than primary fibroblasts after lentiviral transduction. Additionally, HBD-2 secretion from transduced HGECs cells was further increased when stimulated with IL-1 or TNFα. Our data indicate that while HBD-2 expression is limited in primary fibroblasts, its expression in HGECs may be maximized by gene transduction plus cytokine induction.