Diet-Induced Weight Loss and Exercise Alone and in Combination Enhance the Expression of Adiponectin Receptors in Adipose Tissue and Skeletal Muscle, but Only Diet-Induced Weight Loss Enhanced Circulating Adiponectin
Diet-Induced Weight Loss and Exercise Alone and in Combination Enhance the Expression of Adiponectin Receptors in Adipose Tissue and Skeletal Muscle, but Only Diet-Induced Weight Loss Enhanced Circulating Adiponectin
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DOI：
10.1210/jc.2008-2505
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发表时间：
2010-02-01
影响因子：
5.8
通讯作者：
Richelsen, Bjorn
中科院分区：
文献类型：
--
作者：
Christiansen, Tore;Paulsen, Soren K.;Richelsen, Bjorn
目的：本研究旨在探讨减肥和运动单独或联合对低分子量、中分子量和高分子量脂联素循环水平以及脂肪组织（AT）和骨骼肌（SM）中脂联素和脂联素受体（AdipoR）表达的影响。设计与方法：79名肥胖男性和女性随机分为以下组：1)只运动（12周的运动，不限制饮食）；2)低热量饮食[8周的极低能量饮食（600千卡/天），然后4周的体重维持饮食]；3)低热量饮食和运动（DEX； 8周极低能量饮食800千卡/天，然后4周体重维持饮食结合整个12周的运动）。在基线和12周后收集sc腹部AT和SM的血液样本和活检。Western blot法测定血清中脂联素的分子亚型。结果：运动组和DEX组大鼠SM组织中AdipoR1、2 mRNA表达量均显著升高（P < 0.05）。3组AT中脂联素和adipor mRNA表达量均显著升高（P < 0.01），而血清总循环脂联素仅在DEX和低热量饲粮组显著升高（P < 0.01）。所有的脂联素亚形式都以与总脂联素相似的方式发生变化，表明干预对任何亚形式都没有特定的调节。结论：单独运动和结合饮食诱导的减肥可以增强AT和SM中脂联素受体的mRNA表达，但只有明显的低热量诱导的减肥才能增加肥胖受试者的循环脂联素。[J] .中华内分泌杂志，2001,21(5):444 - 444。
Objective: The aim of the study was to investigate the effect of weight loss and exercise independently and in combination on circulating levels of adiponectin including low molecular weight, medium molecular weight, and high molecular weight adiponectin and expression of adiponectin and adiponectin receptors (AdipoR) in adipose tissue (AT) and skeletal muscle (SM).Design and Methods: Seventy-nine obese males and females were randomized into the following: 1) exercise only (12 wk of exercise without diet restriction); 2) hypocaloric diet [8 wk of very low energy diet (600 kcal/d) followed by 4 wk with a weight maintenance diet]; and 3) hypocaloric diet and exercise (DEX; 8 wk very low energy diet 800 kcal/d followed by 4 wk weight maintenance diet combined with exercise throughout the 12 wk). Blood samples and biopsies from sc abdominal AT and SM were collected at baseline and after 12 wk. The molecular subforms of adiponectin in serum were determined by Western blot.Results: The mRNA expression of AdipoR1 and -2 in SM was increased significantly in the exercise-only and DEX groups (both P < 0.05). The mRNA expression of adiponectin and AdipoRs in AT was increased significantly in all three groups (all P < 0.01), whereas serum total circulating adiponectin was significantly increased only in the DEX and hypocaloric diet groups (both P < 0.01). All the adiponectin subforms changed in a similar manner as total adiponectin, indicating no specific regulation of any of the subforms by the intervention.Conclusion: Exercise alone and in combination with a diet-induced weight loss enhance the mRNA expression of adiponectin receptors in AT and in SM but only a pronounced hypocaloric-induced weight-loss increases circulating adiponectin in obese subjects. (J Clin Endocrinol Metab 95: 911-919, 2010)