EFFECTS OF OXYGEN ON AEROSOLIZED SERRATIA MARCESCENS
EFFECTS OF OXYGEN ON AEROSOLIZED SERRATIA MARCESCENS
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DOI：
10.1128/aem.13.5.781-787.1965
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发表时间：
1965-01-01
期刊：
影响因子：
--
通讯作者：
HESS, GE
中科院分区：
文献类型：
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作者：
HESS, GE
粘质链霉菌(ATCC菌株-14041)在水中的悬浮液在不同浓度的氧气存在下，在旋转转鼓中被雾化。在相对湿度(RH)为40%、温度为25℃的条件下，当接触0.25%或更多的氧气时，气雾化微生物的菌落形成能力迅速被破坏，但至少5小时几乎不受影响。在含氧量不超过百万分之十的氮气中。完全水合的生物体对压力高达100磅/平方英寸的氧气4小时不敏感。在相对湿度为97%的空气中，洗涤细胞的气雾剂中没有出现活性损失。有人认为，气雾化细胞的脱水导致了对氧气致死效应的敏化，但不是死亡的主要原因。Mn++、Co++、甘油和硫脲提高了气溶胶在空气中的生物稳定性。在这个系统和使用冻干或辐照生物体或无细胞酶的系统中，氧的影响之间有许多相似之处，支持这一假设，即涉及密切相关的机制。
Suspensions of S. marcescens (ATCC strain-14041) in water were aerosolized in a rotating drum in the presence of various concentrations of oxygen. The colony-forming ability of aerosolized organisms was rapidly destroyed by contact with 0.25% or more oxygen at 40% relative humidity (RH) and 25 C, but was almost unimpaired for at least 5 hr. in nitrogen containing not more than 10 ppm of oxygen. Completely hydrated organisms were insensitive to oxygen at pressures up to 100 psi for 4 hr. No loss in viability occurred in aerosols of washed cells in air at 97% RH. It is proposed that dehydration of the aerosolized cells results in sensitization to lethal effects of oxygen, but is not the primary cause of death. Mn++, Co++, glycerol, and thiourea enhanced the biological stability of aerosols in air. Numerous similarities between the effects of oxygen in this system and in systems using freeze-dried or irradiated organisms or cell-free enzymes support the hypothesis that closely related mechanisms are involved.