IMPROVED NUTRIENT UPTAKE ENHANCES COTTON GROWTH AND SALINITY TOLERANCE IN SALINE MEDIA
IMPROVED NUTRIENT UPTAKE ENHANCES COTTON GROWTH AND SALINITY TOLERANCE IN SALINE MEDIA
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DOI：
10.1080/01904167.2014.881869
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发表时间：
2014-01-01
影响因子：
2.1
通讯作者：
Dong, H. Z.
中科院分区：
文献类型：
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作者：
Dai, J. L.;Duan, L. S.;Dong, H. Z.
通过两个试验来确定营养吸收的改善是否能提高棉花的耐盐性。第一个试验采用耐盐性提高的转基因棉花品系（CMO3）及其野生品系（SM3）在基质（泥炭：蛭石= 1:1,v/v）的盆栽中培养，第二个试验采用分根水培法培养棉花品系（SCRC 28）。测定了盐[NaCl]处理后植物组织中必需营养元素和Na+含量、叶片光合作用（Pn）和叶绿素（Chl）浓度以及植物生物量。在第一个试验中，与未受盐胁迫的对照相比，150 mM NaCl盐胁迫降低了SM3和CMO3的生物量和光合速率（Pn），但CMO3的降低幅度明显低于SM3，表明CMO3的耐盐性相对于SM3有所提高。CMO3对主要营养元素[氮(N)、磷(P)、钾(K)、钙（Ca）、镁（Mg）、铁（Fe）、锰（Mn）、铜（Cu）、锌（Zn）]的总吸收量和含量高于SM3。与SM3相比，CMO3的钠（Na+）积累较少，Na/N、Na/P、Na/K、Na/Ca、Na/Mg、Na/Fe、Na/Mn、Na/Cu和Na/Zn的极值比值也较低。转基因AhCMO棉花耐盐性的提高可能是由于其营养吸收能力优于SM3。在2个试验中，非胁迫根半饲喂中等水平营养液和低水平营养液（CMN/SLN）比盐胁迫根半饲喂中等水平营养液和无胁迫根半饲喂低水平营养液（CLN/SMN）表现出更高的耐盐性。与CLN/SMN相比，CMN/SLN处理下植株吸收的养分更多，但Na+吸收较少。综上所述，改善养分吸收对棉花耐盐性的增强起着重要作用。
Two experiments were conducted to determine if improved nutrient uptake increases salinity tolerance of cotton (Gossypium hirsutum L.). A transgenic cotton line (CMO3) with increased salt tolerance and its wild line (SM3) were grown in pots containing substrate (peat: vermiculite = 1: 1, v/v) in the first experiment, while cotton ('SCRC 28') was cultured in hydroponics with a split-root system in the second experiment. Contents of essential nutrient elements and Na+ in plant tissues, leaf photosynthesis (Pn) and chlorophyll (Chl) concentration and plant biomass were determined after salinity [sodium chloride (NaCl)] treatment in both experiments. In the first experiment, salinity stress with 150 mM NaCl reduced plant biomass and photosynthesis (Pn) of both SM3 and CMO3 compared with their non-stressed controls, but the CMO3 suffered significantly lower reductions than SM3, suggesting an increased salinity tolerance of CMO3 relative to SM3. Total uptake and contents of main nutrient elements [nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), copper (Cu), and zinc (Zn)] in CMO3 were higher than those in SM3. Also, less sodium (Na+) accumulation and lower extreme ratios of Na/N, Na/P, Na/K, Na/Ca, Na/Mg, Na/Fe, Na/Mn, Na/Cu, and Na/Zn were observed in CMO3 than in SM3. Increased salt tolerance in transgenic AhCMO cotton was probably attributed to its superior nutrient uptake compared with SM3. In the second experiment, the non-stressed root half fed with moderate level of nutrient solution and salt-stressed half fed with low level of nutrient solution (CMN/SLN) exhibited higher salinity tolerance than salt-stressed root half fed with moderate level of nutrient solution and non-stressed root half fed with low nutrient solution (CLN/SMN). Plants absorbed more nutrients but less Na+ under CMN/SLN than CLN/SMN. The overall results suggest that improved nutrient uptake played an important role in the enhanced salt tolerance of cotton.