Ex vivo expansion of hematopoietic stem and progenitor cells for transplantation.
Ex vivo expansion of hematopoietic stem and progenitor cells for transplantation.
复制标题
用于移植的造血干细胞和祖细胞的离体扩增。
DOI：
10.1007/978-1-4615-6349-5_8
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发表时间：
1997
影响因子：
--
通讯作者：
Miller,WM
中科院分区：
文献类型：
--
作者：
LaIuppa,JA;Papoutsakis,ET;Miller,WM
Over the past few years, there has been a heightened interest in the development of clinical-scale culture systems for ex vivo expansion of hematopoietic cells.离体扩增的造血细胞可用于多种临床应用，例如移植、肿瘤清除和基因治疗。 The anticipated benefits of ex vivo expanded hematopoietic cells over bone marrow or peripheral blood for transplant patients include a shortened engraftment period following chemotherapy and reduction in the number of harvested bone marrow or peripheral blood progenitor cells required for an adequate autograft.然而，在离体扩增的细胞用于临床之前，需要解决几个关键问题。 The culture conditions and systems used for expansion of these cells will need to be established, since the growth factors and other culture parameters used in the expansion process greatly influence the lineage and maturation stage of the cells produced. These factors are considered below, along with regulatory and safety issues, with the goal of providing a framework for consistent and reproducible ex vivo expansion for clinical application.
Over the past few years, there has been a heightened interest in the development of clinical-scale culture systems for ex vivo expansion of hematopoietic cells. Ex vivo expanded hematopoietic cells are useful for a variety of clinical applications such as transplantation, tumor purging, and gene therapy. The anticipated benefits of ex vivo expanded hematopoietic cells over bone marrow or peripheral blood for transplant patients include a shortened engraftment period following chemotherapy and reduction in the number of harvested bone marrow or peripheral blood progenitor cells required for an adequate autograft. However, several key issues need to be addressed before ex vivo expanded cells can be used clinically. The culture conditions and systems used for expansion of these cells will need to be established, since the growth factors and other culture parameters used in the expansion process greatly influence the lineage and maturation stage of the cells produced. These factors are considered below, along with regulatory and safety issues, with the goal of providing a framework for consistent and reproducible ex vivo expansion for clinical application.