CELL-DEATH OF AXOTOMIZED MOTONEURONS IN NEONATAL RATS, AND ITS PREVENTION BY PERIPHERAL REINNERVATION
CELL-DEATH OF AXOTOMIZED MOTONEURONS IN NEONATAL RATS, AND ITS PREVENTION BY PERIPHERAL REINNERVATION
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DOI：
10.1113/jphysiol.1987.sp016526
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发表时间：
1987-05-01
影响因子：
5.5
通讯作者：
MIYATA, Y
中科院分区：
文献类型：
--
作者：
KASHIHARA, Y;KUNO, M;MIYATA, Y
1. 在出生后第4天，对大鼠近肌内腓肠肌神经进行切开术后运动神经元死亡的研究。2. 运动神经再支配切除腓肠肌内侧神经后获得的最大抽搐张力约为对侧完整侧测得的70%。3. 第4天切除腓肠肌内侧神经的大鼠在35 ~ 45日龄时的运动单位数为正常大鼠的62%。4. 在腓肠肌内侧神经切片后30-40天，用辣根过氧化物酶（HRP）逆行标记的腓肠肌内侧运动神经元的数量是对侧完整侧标记的77%。5. 当第4天切除腓肠肌内侧神经并阻止外周再神经支配时，用HRP标记的腓肠肌内侧运动神经元的数量平均仅为对照组的18%。6. 外周再神经支配预防后，经HRP标记的腓肠肌内侧运动神经元数量减少，运动细胞柱神经元密度降低，提示运动神经元死亡。7. 大多数出生后4天切除的腓肠肌内侧运动神经元在没有靶接触的情况下仍能存活约2周。8. 术后第4天，腓肠肌内侧运动纤维复合动作电位面积一度下降，如果允许周围神经再神经支配，则术后第12天开始恢复，而未给予再神经支配的运动神经元，复合动作电位继续下降。9. 由此可见，先前在胚胎阶段观察到的运动神经元存活的靶依赖性在产后早期仍然存在。
1. Motoneurone death induced by axotomy in the rat was studied following section of the medial gastrocnemius nerve near the muscle 4 days after birth. 2. The maximum twitch tension of the medial gastrocnemius muscle achieved by motor reinnervation after section of its nerve was about 70% of that measured on the contralateral, intact side. 3. The number of motor units counted at 35-45 days of age in the animals whose medial gastrocnemius nerves had been sectioned on day 4 was 62% of that observed in normal rats. 4. The number of medial gastrocnemius motoneurones retrogradely labelled with horseradish peroxidase (HRP) 30-40 days after section of the medial gastrocnemius nerve was 77% of that labelled on the contralateral, intact side. 5. When the medial gastrocnemius nerve had been sectined on day 4 and prevented from peripheral reinnervation, the number of medial gastrocnemius motoneurones labelled with HRP was, on the average, only 18% of that labelled on the control side. 6. Decreased number of medial gastrocnemius motoneurones labelled with HRP following prevention of peripheral reinnervation was associated with a decrease in the neurone density of the motor cell column, indicating that occurrence of motoneurone death. 7. The majority of medial gastrocnemius motoneurones axotomized 4 days after birth appear to maintain their survival for about 2 weeks without target contact. 8. The area of the compound action potential of medial gastrocnemius motor fibers once decreased after axotomy on day 4 began to recover from the 12th day after the operation if reinnervation by the cut peripheral nerve had been allowed, whereas the compound action potential continued to decrease in those axotomized motoneurones whose reinnervation had been prevented. 9. It is concluded that target dependence of motoneurone survival previously observed at embryonic stages is still present during the early post-natal period.