IL-4 Derived from Non-T Cells Induces Basophil- and IL-3-independent Th2 Immune Responses.
IL-4 Derived from Non-T Cells Induces Basophil- and IL-3-independent Th2 Immune Responses.
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DOI：
10.4110/in.2013.13.6.249
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发表时间：
2013-12
期刊：
影响因子：
6
通讯作者：
Min B
中科院分区：
文献类型：
--
作者：
Kim S;Karasuyama H;Lopez AF;Ouyang W;Li X;Le Gros G;Min B
Th 2免疫如何在体内发展仍然不清楚。嗜碱性粒细胞被认为是产生IL-4的关键先天细胞，IL-4是Th 2免疫所必需的细胞因子。越来越多的证据表明，嗜碱性粒细胞是启动Th 2免疫。在这项研究中，我们重新审视了嗜碱性粒细胞在各种类型的佐剂诱导的Th 2免疫应答中的作用。IL-3或IL-3受体缺陷的小鼠，其中嗜碱性淋巴结募集被完全消除，响应于寄生虫抗原或木瓜蛋白酶免疫，完全发展出野生型水平的Th 2 CD 4 T细胞应答。在嗜碱性粒细胞被诱导消融的小鼠中也观察到类似的发现。有趣的是，来自非T细胞的IL-4似乎对产生IL-4的CD 4 T细胞的产生至关重要。其他Th 2促进因子包括IL-25和胸腺基质淋巴细胞生成素（TSLP）均未检测到。因此，我们的研究结果表明，IL-3和嗜碱性粒细胞独立的体内Th 2免疫的发展与非T细胞来源的IL-4的帮助下，提供了一个额外的机制，Th 2型免疫反应在体内出现。
How Th2 immunity develops in vivo remains obscure. Basophils have been considered key innate cells producing IL-4, a cytokine essential for Th2 immunity. Increasing evidence suggests that basophils are dispensable for the initiation of Th2 immunity. In this study, we revisited the role of basophils in Th2 immune responses induced by various types of adjuvants. Mice deficient in IL-3 or IL-3 receptor, in which basophil lymph node recruitment is completely abolished, fully developed wild type level Th2 CD4 T cell responses in response to parasite antigen or papain immunization. Similar finding was also observed in mice where basophils are inducibly ablated. Interestingly, IL-4-derived from non-T cells appeared to be critical for the generation of IL-4-producing CD4 T cells. Other Th2 promoting factors including IL-25 and thymic stromal lymphopoietin (TSLP) were dispensable. Therefore, our results suggest that IL-3- and basophil-independent in vivo Th2 immunity develops with the help of non-T cell-derived IL-4, offering an additional mechanism by which Th2 type immune responses arise in vivo.