Dynamic imaging of fibrin network formation correlated with other measures of polymerization
Dynamic imaging of fibrin network formation correlated with other measures of polymerization
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DOI：
10.1182/blood-2007-08-105247
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发表时间：
2008-05-15
期刊：
影响因子：
20.3
通讯作者：
Weisel, John W.
中科院分区：
文献类型：
--
作者：
Chernysh, Irina N.;Weisel, John W.
利用反褶积显微镜，我们实时观察了水合状态下纤维蛋白网络的形成。在凝胶点前观察单个移动纤维。凝胶化后，可以看到一个初始的纤维蛋白网络，随着时间的推移，新纤维的加入和其他纤维的延伸和分支而进化。此外，网络中的一些光纤移动了一段时间。我们通过分支点的数量以及纵向和横向生长纤维来量化网络的形成。80%的支点形成，70%的纤维在凝胶点达到最大长度。相反，在凝胶点，以荧光强度测量的纤维直径小于完全形成的凝块最大值的25%，浊度小于15%。达到最终长度的纤维累积百分比和分支点数量在最大浊度的60%时达到最大值。侧纤维的生长与浊度同时达到平稳。对凝块力学性能的测量表明，在结构参数达到最大值后，凝块达到最大刚度和最小塑性。这些结果为纤维蛋白网络形成过程中事件的相对时间序列提供了新的信息。
Using deconvolution microscopy, we visualized in real time fibrin network formation in the hydrated state. Individual mobile fibers were observed before the gel point determined by eye. After gelation, an initial fibrin network was seen, which evolved over time by addition of new fibers and elongation and branching of others. Furthermore, some fibers in the network moved for a time. We quantified network formation by number of branch points, and longitudinal and lateral growth fibers. Eighty percent of branch points were formed, and 70% of all fibers reached their maximum length at the gel point. In contrast, at the gel point, fiber diameter, measured as fluorescence intensity, was less than 25% and turbidity was less than 15% of the maximum values of the fully formed clot. The cumulative percentage of fibers reaching their final length and the number of branch points attained maximum values at 60% of maximum turbidity. Lateral fiber growth reached a plateau at the same time as turbidity. Measurements of clot mechanical properties revealed that the clots achieved maximum stiffness and minimum plasticity after the structural parameters reached their maxima. These results provide new information on the relative time sequence of events during fibrin network formation.