The Effect of Pre-Condition Cerebella Fastigial Nucleus Electrical Stimulation within and beyond the Time Window of Thrombolytic on Ischemic Stroke in the Rats
The Effect of Pre-Condition Cerebella Fastigial Nucleus Electrical Stimulation within and beyond the Time Window of Thrombolytic on Ischemic Stroke in the Rats
复制标题
溶栓时间窗内和之外的先决条件小脑顶核电刺激对大鼠缺血性中风的影响。
DOI：
10.1371/journal.pone.0128447
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发表时间：
2015-05-27
期刊：
影响因子：
3.7
通讯作者：
Huang, Wen
中科院分区：
文献类型：
--
作者：
Tang, Weiju;Dong, Weiwei;Huang, Wen
目的探讨电刺激小脑顶核（FNS）对大鼠脑缺血再灌注损伤的神经保护作用及过氧化物酶体增殖物激活物受体（PPAR）γ介导的炎症反应的作用。再灌注12、15 h后静脉注射重组组织型纤溶酶原激活剂（rt-PA），对照组不注射FNS。再灌注72 h后，观察神经功能缺损、脑梗死体积和脑水肿情况。采用分光光度计测定缺血半暗带脑组织髓过氧化物酶（MPO）活性及过氧化物酶体增殖物激活受体（PPAR）表达。结果与对照组比较，FNS组脑梗死体积、脑水肿明显减轻，神经功能缺损程度明显改善，尤以再灌注6 h和9 h组为显著（P < 0.05）。
ObjectiveTo investigate the effect of neurogenic neuroprotection conferred by cerebellar fastigial nucleus stimulation (FNS) and the role of PPAR gamma-mediated inflammation in a rat model of cerebral ischemia reperfusion.MethodsAfter a continuous 1 hour fastigial nucleus electric stimulation, the male Sprague Dawley (SD) rats were given middle cerebral artery occlusion (MCAO) for 1, 3, 6, 9, 12 and 15 hours undergoing reperfusion with intravenous recombinant tissue plasminogen activator (rt-PA), while the control group received without FNS. After 72h of reperfusion, the neurological deficits, infarct volume and brain edema were evaluated. The brain tissue in ischemic penumbra was determined the myeloperoxidase (MPO) activity by a spectrophotometer and expression of PPAR. was measured by Rt-PCR and Western blotting.ResultsOur findings showed that FNS group had significantly reduced infarct volume and brain edema, and improved neurological deficits compared with the control group, especially in 6h and 9h reperfusion subgroups(p