How B-Cell Receptor Repertoire Sequencing Can Be Enriched with Structural Antibody Data.
How B-Cell Receptor Repertoire Sequencing Can Be Enriched with Structural Antibody Data.
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DOI：
10.3389/fimmu.2017.01753
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发表时间：
2017
影响因子：
7.3
通讯作者：
Trück J
中科院分区：
文献类型：
--
作者：
Kovaltsuk A;Krawczyk K;Galson JD;Kelly DF;Deane CM;Trück J
免疫球蛋白基因库的下一代测序 (Ig-seq) 允许在序列水平上研究大规模抗体动态。然而，结构信息（抗体结合能力的关键描述符）并未在 Ig-seq 协议中收集。在 Ig-seq 收集的抗体序列信息与 X 射线晶体学等低通量技术之间建立系统关系可以从根本上提高我们对抗体的理解。 Ig-seq 数据集与已知抗体结构的映射可以在结构上（或许在功能上）指示未知区域。此外，使用结构抗体描述符对比原始数据集和抗原挑战数据集应该可以提供对抗体成熟的见解。随着抗体结构数量的稳步增加以及越来越多的 Ig-seq 数据集的出现，结合两类信息所产生的机会也随之增加。在这里，我们回顾这些数据类型如何相互丰富，并显示出提高我们对免疫系统的知识和改进抗体工程的潜力。
Next-generation sequencing of immunoglobulin gene repertoires (Ig-seq) allows the investigation of large-scale antibody dynamics at a sequence level. However, structural information, a crucial descriptor of antibody binding capability, is not collected in Ig-seq protocols. Developing systematic relationships between the antibody sequence information gathered from Ig-seq and low-throughput techniques such as X-ray crystallography could radically improve our understanding of antibodies. The mapping of Ig-seq datasets to known antibody structures can indicate structurally, and perhaps functionally, uncharted areas. Furthermore, contrasting naïve and antigenically challenged datasets using structural antibody descriptors should provide insights into antibody maturation. As the number of antibody structures steadily increases and more and more Ig-seq datasets become available, the opportunities that arise from combining the two types of information increase as well. Here, we review how these data types enrich one another and show potential for advancing our knowledge of the immune system and improving antibody engineering.