Preparation of Zn-doped β-tricalcium phosphate (β-Ca3(PO4)2) bioceramics
Preparation of Zn-doped β-tricalcium phosphate (β-Ca3(PO4)2) bioceramics
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DOI：
10.1016/j.msec.2006.05.051
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发表时间：
2007-04
期刊：
影响因子：
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通讯作者：
A. Tas;S. Bhaduri;S. Jalota
中科院分区：
文献类型：
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作者：
A. Tas;S. Bhaduri;S. Jalota
采用湿法化学/共沉淀法制备了纯β-磷酸三钙(β-TCP)和掺锌(600,2900,4100,7000,9300和10,100ppm)β-TCP样品，然后在空气中于1000℃下焙烧。共沉淀法的前驱体粉末为缺钙纳米磷灰石(即Ca/P摩尔比在1.49~1.51之间变化)，具有针状但团聚的颗粒，厚度为30 nm。小鼠成骨样细胞的体外培养实验表明，锌掺杂2900~4100ppm的样品具有最高的细胞活力(通过活/死计数)，并且随着锌含量的进一步增加，孔板中的死亡细胞数量开始增加。掺入4100nmZn的β-TcP样品的碱性磷酸酶活性达到峰值。通过非接触轮廓术测量的样品表面粗糙度也对活/死细胞计数有影响，本研究中遇到的最高细胞存活率对应于粗糙度最小的表面。
Pure β-tricalcium phosphate (β-TCP) and Zn-doped (600, 2900, 4100, 7000, 9300 and 10,100 ppm) β-TCP samples were prepared by using a wet chemical/coprecipitation synthesis technique, followed by calcination at 1000 °C in air. Precursor powders of the coprecipitation process were Ca-deficient nanoapatites (i.e., Ca/P molar ratio varying from 1.49 to 1.51) with needlelike but agglomerated particles of 30 nm thickness. In vitro culture tests performed by mouse osteoblast-like cells showed that the samples doped with 2900 to 4100 ppm Zn showed the highest cell viability (via Live/Dead counts), and with a further increase in the Zn-content towards 1 wt.% the number of dead cells in the well plates started to increase. Alkaline phosphatase (ALP) activity peaked for the β-TCP sample doped with 4100 pm Zn. The sample surface roughness, measured by non-contact profilometry, was also found to have an effect on the Live/Dead cell counts, and the highest cell viability encountered in this study corresponded to the surface with the least roughness.