Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure.
Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure.
复制标题
使用负压和正压通过脉管系统和气道对完整肺组织进行脱细胞。
DOI:
10.1007/7651_2017_32
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发表时间:
2018
期刊:
影响因子:
--
通讯作者:
Panoskaltsis-Mortari,Angela
中科院分区:
文献类型:
--
作者:
Skolasinski,Steven;Panoskaltsis-Mortari,Angela
Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.