Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure.

Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure.
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使用负压和正压通过脉管系统和气道对完整肺组织进行脱细胞。

DOI:
10.1007/7651_2017_32
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发表时间:
2018
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
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通讯作者:
Panoskaltsis-Mortari,Angela
Panoskaltsis-Mortari,Angela
中科院分区:
--
文献类型:
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作者:
Skolasinski,Steven;Panoskaltsis-Mortari,Angela

文献摘要

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脱细胞可以生产细胞外基质(ECM)支架。在这里,我们描述了使用正压和负压组合将脱细胞试剂分别驱动到结构完整的肺的脉管系统和气道中,以去除细胞和细胞材料,留下完整的 ECM 支架。
Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.