Improved method for the measurement of glutamate and aspartate using capillary electrophoresis with laser induced fluorescence detection and its application to brain microdialysis
Improved method for the measurement of glutamate and aspartate using capillary electrophoresis with laser induced fluorescence detection and its application to brain microdialysis
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DOI：
10.1016/s0378-4347(97)00146-1
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发表时间：
1997-07-04
期刊：
影响因子：
--
通讯作者：
Palmer, AM
中科院分区：
文献类型：
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作者：
Dawson, LA;Stow, JM;Palmer, AM
我们以前发表的数据分析谷氨酸在微透析样品中使用市售CE装置。在这里，我们展示了进一步的改进，从非常小的体积微透析液中的谷氨酸和天冬氨酸的分析。我们的系统的检测限已增加到10（-9）M的谷氨酸和天冬氨酸。这使得微透析采样时间减少到2分钟，从而提高了微透析采样的时间分辨率。同时，迁移时间也已减少，使这两种氨基酸的分辨率可以在2分钟内实现。这种新的分析方法已被应用到测量的EAA从海马齿状回的微透析样品。细胞外的谷氨酸和天冬氨酸的浓度增加到最大的5-和4.5倍的预输液值，分别在输液100 mM的K+通过微透析探针。这与去极化诱发的这两种氨基酸从该脑区域的释放是一致的。(C)1997年Elsevier Science B.V.
We have previously published data on the analysis of glutamate in microdialysis samples using a commercially availble CE apparatus. Here we demonstrate further improvements in the analysis of both glutamate and aspartate from very small volume microdialysates. The limit of detection of our system has been increased to 10(-9) M for both glutamate and aspartate. This permits microdialysis sampling time to be reduced to 2 min, thus improving the temporal resolution of microdialysis sampling. Concurrently, migration time has also been reduced such that resolution of both amino acids can be achieved inside 2 min. This new analytical method has been applied to the measurement of the EAA from microdialysis samples from the dentate gyrus of the hippocampus. Extracellular concentrations of both glutamate and aspartate increased to a maximum of 5- and 4.5-fold of preinfusion values, respectively, during infusion of 100 mM K+ through the microdialysis probe. This is consistent with the depolarization-evoked release of both amino acids from this brain region. (C) 1997 Elsevier Science B.V.