Behavioural and neuronal activation after microinjections of AMPA and NMDA into the perifornical lateral hypothalamus in rats
Behavioural and neuronal activation after microinjections of AMPA and NMDA into the perifornical lateral hypothalamus in rats
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DOI：
10.1016/j.bbr.2011.06.021
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发表时间：
2011-10-31
影响因子：
2.7
通讯作者：
Semba, Kazue
中科院分区：
文献类型：
--
作者：
Li, Frederick W.;Deurveilher, Samuel;Semba, Kazue
下丘脑穹窿周围外侧区（Perfornicallateralhypothalamicarea，PeFLH）是下丘脑食欲素/下丘脑泌素（orexin/hypocretin，OX）神经元的主要分布区，在唤醒、摄食和运动活动中起重要作用。本研究探讨了行为的影响，激活PeFLH神经元与微量注射离子型谷氨酸受体激动剂。将（1）AMPA（1和2 mM溶于0.1 μ L人工脑脊液，ACSF）和ACSF，或（2）NMDA（1和10 mM溶于0.1 μ L ACSF）和ACSF分别单侧微量注射到成年雄性大鼠的PeFLH中。每次注射后，将大鼠置于开阔视野中进行行为评分45分钟。第三次注射后对大鼠进行灌注，进行c-Fos和OX免疫组织化学，以评估OX神经元的激活水平。行为分析表明，与ACSF条件相比，AMPA注射产生了剂量依赖性的运动和饲养增加，持续整个45分钟的记录期间，并增加饮酒。注射NMDA在10 mM，但不是1 mM，诱导运动的短暂增加和增加喂养。组织学分析表明，虽然这两种激动剂增加了PeFLH中c-Fos免疫反应性神经元的数量，但只有AMPA增加了c-Fos和OX免疫反应性神经元的数量。c-Fos/OX免疫反应阳性神经元的数量与运动量、直立量和饮水量呈正相关。这些结果支持OX和PeFLH中其他神经元上的离子型谷氨酸受体在运动和摄食行为调节中的作用。(C)2011 Elsevier B. V.保留所有权利。
The perifornical lateral hypothalamic area (PeFLH), which houses orexin/hypocretin (OX) neurons, is thought to play an important role in arousal, feeding, and locomotor activity. The present study examined behavioural effects of activating PeFLH neurons with microinjections of ionotropic glutamate receptor agonists. Three separate unilateral microinjections of either (1) AMPA (1 and 2 mM in 0.1 mu L artificial cerebrospinal fluid, ACSF) and ACSF, or (2) NMDA (1 and 10 mM in 0.1 mu L ACSF), and ACSF were made into the PeFLH of adult male rats. Following each injection, the rats were placed into an open field for behavioural scoring for 45 min. Rats were perfused after the third injection for immunohistochemistry for c-Fos and OX to assess the level of activation of OX neurons. Behavioural analyses showed that, as compared to ACSF conditions, AMPA injections produced a dose-dependent increase in locomotion and rearing that persisted throughout the 45 min recording period, and an increase in drinking. Injection of NMDA at 10 mM, but not 1 mM, induced a transient increase in locomotion and an increase in feeding. Histological analyses showed that while both agonists increased the number of neurons immunoreactive for c-Fos in the PeFLH, only AMPA increased the number of neurons immunoreactive for both c-Fos and OX. There were positive correlations between the number of c-Fos/OX-immunoreactive neurons and the amounts of locomotion, rearing, and drinking. These results support the role of ionotropic glutamate receptors on OX and other neurons in the PeFLH in the regulation of locomotor and ingestive behaviours. (C) 2011 Elsevier B.V. All rights reserved.