FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF A NOVEL RECEPTOR FOR VASOACTIVE INTESTINAL POLYPEPTIDE
FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF A NOVEL RECEPTOR FOR VASOACTIVE INTESTINAL POLYPEPTIDE
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DOI：
10.1016/0896-6273(92)90101-i
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发表时间：
1992-04-01
期刊：
影响因子：
16.2
通讯作者：
NAGATA, S
中科院分区：
文献类型：
--
作者：
ISHIHARA, T;SHIGEMOTO, R;NAGATA, S
血管活性肠肽(VIP)是一种由28个氨基酸组成的多肽激素，在外周和中枢神经系统中发挥多种生理作用。通过与分泌素受体基因的杂交，从大鼠肺组织的cDNA文库中获得了VIP受体的功能基因克隆。VIP结合在小鼠COP细胞中表达的VIP受体，并通过该受体激活腺苷环化酶。大鼠血管活性肠肽受体由459个氨基酸组成，M(R)为52,054，包含7个跨膜片段。它在结构上与促胰液素、降钙素和甲状旁腺激素受体相关，提示它们构成了G(S)蛋白偶联受体的一个新的亚家族。VIP受体在大鼠肝、肺、肠、脑等组织中均有表达。原位杂交显示，VIP受体mRNA广泛分布于成年大鼠脑神经细胞，在大脑皮层和海马区表达相对较高。
Vasoactive intestinal polypeptide (VIP), a 28 amino acid peptide hormone, plays many physiological roles in the peripheral and central nerve systems. A functional cDNA clone of the VIP receptor was isolated from a rat lung cDNA library by cross-hybridization with the secretin receptor cDNA. VIP bound the cloned VIP receptor expressed in mouse COP cells and stimulated adenylate cyclase through the cloned receptor. The rat VIP receptor consists of 459 amino acids with a calculated M(r) of 52,054 and contains seven transmembrane segments. It is structurally related to the secretin, calcitonin, and parathyroid hormone receptors, suggesting that they constitute a new subfamily of the G(s) protein-coupled receptors. VIP receptor mRNA was detected in various rat tissues including liver, lung, intestines, and brain. In situ hybridization revealed that VIP receptor mRNA is widely distributed in neuronal cells of the adult rat brain, with a relatively high expression in the cerebral cortex and hippocampus.