Genetic characterization and phylogenetic position of Echinococcus felidis Ortlepp, 1937 (Cestoda: Taeniidae) from the African lion
Genetic characterization and phylogenetic position of Echinococcus felidis Ortlepp, 1937 (Cestoda: Taeniidae) from the African lion
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DOI：
10.1016/j.ijpara.2007.10.013
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发表时间：
2008-06-01
影响因子：
4
通讯作者：
Ito, Akira
中科院分区：
文献类型：
--
作者：
Huettner, Marion;Nakao, Minoru;Ito, Akira
猫状棘球绦虫于1937年在非洲狮子中被描述，但后来被列为细粒棘球绦虫的一个亚种或一个菌株。在缺乏任何遗传特征的情况下，由于缺乏诊断标准以及可能与共域的细粒棘球绦虫、奥尔特勒皮棘球绦虫和加拿大棘球绦虫混淆，以前来自各种大型非洲哺乳动物的大多数分类群的记录仍未得到证实。在这项研究中，我们从乌干达的狮子粪便中获得了带绦虫卵，并从单个卵子中扩增了DNA。线粒体和核DNA序列与其他棘球绦虫相似，但线粒体基因的高差异值表明存在一个不同的物种。在第二步中，我们将这种材料与大约40年前在南非发现的成年细粒棘球绦虫的保存标本进行了比较。所有DNA片段(
Echinococcus felidis had been described in 1937 from African lions, but was later included in Echinococcus granulosus as a subspecies or a strain. In the absence of any genetic characterization, most previous records of this taxon from a variety of large African mammals remained unconfirmed due to the lack of diagnostic criteria and the possible confusion with the sympatric E granulosus sensu stricto, Echinococcus ortleppi and Echinococcus canadensis. In this study, we obtained taeniid eggs from lion feces in Uganda and amplified DNA from individual eggs. Mitochondrial and nuclear DNA sequences showed similarities with those of other Echinococcus spp., but high values of percentage divergence of mitochondrial genes indicated the presence of a distinct species. In a second step, we compared this material with the preserved specimens of adult E. granulosus felidis, which had been identified morphologically approximately 40 years ago in South Africa. All DNA fragments (