Tear Oxygen Under Hydrogel and Silicone Hydrogel Contact Lenses in Humans
Tear Oxygen Under Hydrogel and Silicone Hydrogel Contact Lenses in Humans
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DOI：
10.1097/opx.0b013e3181b2f582
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发表时间：
2009-08-01
影响因子：
1.4
通讯作者：
Alvord, Larry
中科院分区：
文献类型：
--
作者：
Bonanno, Joseph A.;Clark, Christopher;Alvord, Larry
目的。测定各种常规和硅酮水凝胶隐形眼镜下人体受试者的撕裂氧张力。3个水凝胶透镜和5个硅水凝胶透镜（Dk/t = 17 ~ 329）被氧敏感的牛血清白蛋白- pd中四（4-羧基苯基）卟啉配合物（BSA-porphine）涂覆在背面。采用BSA-porphine氧敏磷光衰减法测定15例非隐形眼镜配戴者的右眼上各类型晶状体的稳态睁开眼泪液氧张力。通过测量闭眼5分钟后泪液氧张力的变化来估计闭眼氧张力。在单独的实验中，在佩戴晶状体的眼睛上放置一个护目镜，并在晶状体上流动一种气体混合物（PO, = 51 torr）来模拟闭眼时晶状体前氧张力。平均睁眼氧压为58 ~ 133托。闭着眼睛的估计范围从1.1到4.2度不等。护目镜下的氧张力在8 ~ 48托之间，8个晶状体中有6个高于闭眼时的估计，表明闭眼前晶状体表面平均氧张力为30，泪液氧张力的测量值明显低于以往研究的预测值。磷光衰减方法能够在不干扰隐形眼镜或角膜的情况下直接测量高Dk/t镜片的体内晶状体后PO2。我们的数据表明，增加Dk/t达到或超过140会继续增加进入中央角膜的通量。（光学视觉科学2009；86:936-942）
Purpose. To determine the tear oxygen tension under a variety of conventional and silicone hydrogel contact lenses in human subjects.Methods. Three hydrogel and five silicone hydrogel lenses (Dk/t = 17 to 329) were coated on the back surface with an oxygen sensitive, bovine serum albumin-Pd meso-tetra (4-carboxyphenyl) porphine complex (BSA-porphine). Each lens type was placed on the right eye of 15 non-contact lens wearers to obtain a steady-state open eye tear oxygen tension using oxygen sensitive phosphorescence decay of BSA-porphine. A closed-eye oxygen tension estimate was obtained by measuring the change in tear oxygen tension after 5 min of eye closure. In separate experiments, a goggle was placed over the lens wearing eye and a gas mixture (PO, = 51 torr) flowed over the lens to Simulate anterior lens oxygen tension during eye closure.Results. Mean open eye oxygen tension ranged from 58 to 133 torr. Closed eye estimates ranged from I I to 42 torr. Oxygen tension under the goggle ranged from 8 to 48 torr and was higher than the closed eye estimate for six out of the eight lenses, suggesting that the average closed eye anterior lens surface oxygen tension is 30, the measured tear oxygen tension is significantly lower than that predicted from previous studies.Conclusions. The phosphorescence decay methodology is capable of directly measuring the in vivo post lens PO2 of high Dk/t lenses without disturbing the contact lens or cornea. Our data indicate that increasing Dk/t up to and beyond 140 continues to yield increased flux into the central cornea. (Optom Vis Sci 2009;86:936-942)