Pinpoint: trajectory planning for multi-probe electrophysiology and injections in an interactive web-based 3D environment.
Pinpoint: trajectory planning for multi-probe electrophysiology and injections in an interactive web-based 3D environment.
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Pinpoint：在基于网络的交互式 3D 环境中进行多探头电生理学和注射的轨迹规划。
DOI：
10.1101/2023.07.14.548952
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Steinmetz,NicholasA
中科院分区：
文献类型：
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作者：
Birman,Daniel;Yang,KennethJ;West,StevenJ;Karsh,Bill;Browning,Yoni;InternationalBrainLaboratory;Siegle,JoshuaH;Steinmetz,NicholasA
在电生理学和注射过程中靶向脑深部结构需要密集的培训和专业知识。即使有经验，研究人员通常也不能确定探针是否精确地放置在目标位置，这种复杂性与实验中同时使用的探针数量有关。在这里，我们提出了Pinpoint，开源软件，允许立体定位插入计划的互动式探索。一旦创建了插入计划，Pinpoint允许用户在线保存这些计划并与合作者共享。3D建模工具允许用户探索他们的插入以及钻机和植入硬件，并确保计划在物理上是可行的。Pinpoint中的探针可以连接到电子微操纵器，从而可以实时可视化当前的大脑区域目标以及神经数据。此外，Pinpoint还可以控制机械手，使插入过程自动化和并行化。与以前可用的软件相比，Pinpoint通过Web浏览器的轻松访问，广泛的功能和实时实验集成可以实现更高效和可重复的记录。
Targeting deep brain structures during electrophysiology and injections requires intensive training and expertise. Even with experience, researchers often can’t be certain that a probe is placed precisely in a target location and this complexity scales with the number of simultaneous probes used in an experiment. Here, we present Pinpoint, open-source software that allows for interactive exploration of stereotaxic insertion plans. Once an insertion plan is created, Pinpoint allows users to save these online and share them with collaborators. 3D modeling tools allow users to explore their insertions alongside rig and implant hardware and ensure plans are physically possible. Probes in Pinpoint can be linked to electronic micro-manipulators allowing real-time visualization of current brain region targets alongside neural data. In addition, Pinpoint can control manipulators to automate and parallelize the insertion process. Compared to previously available software, Pinpoint’s easy access through web browsers, extensive features, and real-time experiment integration enable more efficient and reproducible recordings.