Rheotaxis of Larval Zebrafish: Behavioral Study of a Multi-Sensory Process.
Rheotaxis of Larval Zebrafish: Behavioral Study of a Multi-Sensory Process.
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DOI：
10.3389/fnsys.2016.00014
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发表时间：
2016
影响因子：
3
通讯作者：
Candelier R
中科院分区：
文献类型：
--
作者：
Olive R;Wolf S;Dubreuil A;Bormuth V;Debrégeas G;Candelier R
清醒的动物不断地感知性质和强度有很大变化的感觉输入，了解神经系统如何管理这种持续的不同信息流以获得环境的连贯表示可以说是系统神经科学的一个中心问题。流变性是大多数水生物种共有的朝向水流和游泳保持位置的能力，是一种与生俱来的强大的多感官行为，已知涉及侧线和视觉系统。为了便于斑马鱼幼体流变行为的神经行为学研究，我们开发了一种自由游动幼体的测试方法，该方法允许高实验吞吐量、大统计和对行为的精细描述。我们发现，从探索游泳到逆流游泳存在明显的转变，通过改变鱼类可获得的感觉模式(仅限于视觉、仅限于侧线或两者都有)，并比较不同年龄的游泳模式，我们能够检测和表征两种不同的位置保持机制，一种是由侧线调节的，另一种是由视觉系统调节的。我们还发现，当两种感觉模式都可以访问时，视觉系统使侧线黯然失色，这表明在幼虫阶段，感觉输入不会被合并来微调行为，但多余的信息路径可能被用作功能后备。
Awake animals unceasingly perceive sensory inputs with great variability of nature and intensity, and understanding how the nervous system manages this continuous flow of diverse information to get a coherent representation of the environment is arguably a central question in systems neuroscience. Rheotaxis, the ability shared by most aquatic species to orient toward a current and swim to hold position, is an innate and robust multi-sensory behavior that is known to involve the lateral line and visual systems. To facilitate the neuroethological study of rheotaxic behavior in larval zebrafish we developed an assay for freely swimming larvae that allows for high experimental throughtput, large statistic and a fine description of the behavior. We show that there exist a clear transition from exploration to counterflow swim, and by changing the sensory modalities accessible to the fishes (visual only, lateral line only or both) and comparing the swim patterns at different ages we were able to detect and characterize two different mechanisms for position holding, one mediated by the lateral line and one mediated by the visual system. We also found that when both sensory modalities are accessible the visual system overshadows the lateral line, suggesting that at the larval stage the sensory inputs are not merged to finely tune the behavior but that redundant information pathways may be used as functional fallbacks.