Cytotoxic effects of Coptis chinensis and Epimedium sagittatum extracts and their major constituents (berberine, coptisine and icariin) on hepatoma and leukaemia cell growth
Cytotoxic effects of Coptis chinensis and Epimedium sagittatum extracts and their major constituents (berberine, coptisine and icariin) on hepatoma and leukaemia cell growth
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DOI：
10.1111/j.1440-1681.2004.03951.x
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发表时间：
2004-01-01
影响因子：
2.9
通讯作者：
Chiang, LC
中科院分区：
文献类型：
--
作者：
Lin, CC;Ng, LT;Chiang, LC
1.本研究旨在探讨黄连和箭叶淫羊藿提取物及其主要成分对肝癌和白血病细胞的体外细胞毒作用.本研究采用四种人肝癌细胞系HepG 2、Hep 3B、SK-Hep 1和PLC/PRF/5，以及四种白血病细胞系K562、U937、P3 H1和Raji.两种药材中，C.结果表明，中华绒螯蟹对SK-Hep 1（IC 50 = 7 μ g/mL）和Raji（IC 50 = 4 μ g/mL）细胞系的活性最强。C.对HepG 2、Hep 3B和PLC/PRF/5细胞系的作用浓度分别为20、55和35 μ g/mL。C.对K562、U937和P3 H1细胞株的抑制率分别为29、29和31 μ g/mL.除HepG 2和Hep 3B外，E.箭叶草提取物抑制所有细胞系（SK-Hep 1、PLC/PRF/5、K562、U937、P3 H1和Raji）的增殖，IC 50值分别为15、57、74、221、40和80 μ g/mL。有趣的是，C.黄连碱、小檗碱和黄连碱对肝癌和白血病细胞系的增殖都显示出强烈的抑制作用，IC 50值分别为1.4 - 15.2 μ g/mL和0.6 - 14.1 μ g/mL。然而，淫羊藿苷（E.慈姑）对肝癌或白血病细胞系均无抑制作用。6.本研究结果表明，C。黄连提取物及其主要成分小檗碱和黄连碱具有抗肝癌和抗白血病活性。
1. The present study was conducted to evaluate the cytotoxic effects of Coptis chinensis and Epimedium sagittatum extracts and their major constituents on hepatoma and leukaemia cells in vitro.2. Four human liver cancer cell lines, namely HepG2, Hep3B, SK-Hep1 and PLC/PRF/5, and four leukaemia cell lines, namely K562, U937, P3H1 and Raji, were used in the present study.3. Of the two crude drugs, C. chinensis exhibited the strongest activity against SK-Hep1 (IC50 = 7 mug/mL) and Raji (IC50 = 4 mug/mL) cell lines. The IC50 values for C. chinensis on HepG2, Hep3B and PLC/PRF/5 cell lines were 20, 55 and 35 mug/mL, respectively. The IC50 values for C. chinensis on K562, U937 and P3H1 cell lines were 29, 29 and 31 mug/mL, respectively.4. With the exception of HepG2 and Hep3B, the E. sagittatum extract inhibited the proliferation of all cell lines (SK-Hep1, PLC/PRF/5, K562, U937, P3H1 and Raji), with IC50 values of 15, 57, 74, 221, 40 and 80 mug/mL, respectively.5. Interestingly, the two major compounds of C. chinensis, berberine and coptisine, showed a strong inhibition on the proliferation of both hepatoma and leukaemia cell lines, with IC50 values varying from 1.4 to 15.2 mug/mL and from 0.6 to 14.1 mug/mL, respectively. However, icariin (the major compound of E. sagittatum) showed no inhibition of either the hepatoma or leukaemia cell lines.6. The results of the present study suggest that the C. chinensis extract and its major constituents berberine and coptisine possess active antihepatoma and antileukaemia activities.