Induction of quinone reductase activity by psoralidin isolated from Psoralea corylifolia in mouse hepa 1c1c7 cells
Induction of quinone reductase activity by psoralidin isolated from Psoralea corylifolia in mouse hepa 1c1c7 cells
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DOI：
10.1007/s12272-009-1712-1
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发表时间：
2009-07-01
影响因子：
6.7
通讯作者：
Mar, Woongchon
中科院分区：
文献类型：
--
作者：
Lee, Sung-Jin;Nam, Kung-Woo;Mar, Woongchon
醌还原酶（Quinone reductase，QR）是植物体内一种抗诱变剂和致癌剂的II相保护酶，可被多种化合物诱导。本研究旨在探讨补骨脂种子提取物对Hepa 1c 1c 7小鼠肝癌细胞QR的诱导作用。发现来自种子的甲醇提取物的乙酸乙酯可溶性级分诱导QR，并且1.5倍QR诱导的浓度（1.5 FIC）为1.2 μ g/mL。我们得到一种活性化合物，peptidin，它是在用柱色谱进一步连续分级分离后从乙酸乙酯可溶性级分中分离出来的，peptidin的1.5 FIC为0.5 μ g/mL。杏鲍菇种子和杏鲍菇素可能是开发QR诱导剂的候选物质。
Quinone reductase (QR) is a protective phase II enzyme against mutagens and carcinogens which is inducible by a number of chemical compounds in plants. This study was carried out to investigate effects of the fractions from the seeds of Psoralea corylifolia on the induction of QR with Hepa 1c1c7 murine hepatoma cell line. The ethyl acetate-soluble fraction of the methanolic extract from the seeds was found to induce QR and the concentration of 1.5 fold QR induction (1.5 FIC) was 1.2 mu g/mL. We obtained as an active compound, psoralidin, isolated from the ethyl acetate-soluble fraction after further sequential fractionation with column chromatography and 1.5 FIC of psoralidin was 0.5 mu g/mL. The seeds of Psoralea corylifolia and psoralidin might be a candidate for developing QR inducers.