RADICAL OXIDATION REACTIONS OF THE PURINE MOIETY OF 2'-DEOXYRIBONUCLEOSIDES AND DNA BY IRON-CONTAINING MINERALS
RADICAL OXIDATION REACTIONS OF THE PURINE MOIETY OF 2'-DEOXYRIBONUCLEOSIDES AND DNA BY IRON-CONTAINING MINERALS
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DOI：
10.1093/carcin/14.1.41
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发表时间：
1993-01-01
期刊：
影响因子：
4.7
通讯作者：
CADET, J
中科院分区：
文献类型：
--
作者：
BERGER, M;DEHAZEN, M;CADET, J
采用双实验法研究了黑云母、赤铁矿、磁铁矿、褐铁矿、线铁矿、黄铁矿、蓝铁矿和两种纤蛇纹石（石棉）的自由基氧化能力。一个涉及的电子自旋共振自旋捕获测量的有机自由基的反应得到的活性氧物种，释放后孵育的矿物在磷酸盐缓冲溶液中的甲酸盐作为目标分子。此外，通过使用特异性和灵敏的HPLC电化学测定，在核苷和DNA中搜索矿物质介导的氧化嘌呤分解产物（包括7，8-二氢-8-氧代-2 '-脱氧鸟苷和7，8-二氢-8-氧代-2'-脱氧腺苷）的形成。重点放在两个C（8）羟基嘌呤分解产物的形成所涉及的自由基氧化反应的机理方面。
The radical oxidation capability of several classes of iron minerals, including biotite, hematite, magnetite, minette, nemalite, pyrite, vivianite and two chrysotiles (asbestos), was investigated by using a double experimental approach. One involved the electron spin resonance spin-trapping measurement of organic radicals obtained by the reaction of activated oxygen species, released upon incubation of the minerals in phosphate buffered solutions with formate used as the target molecule. In addition, the formation of mineral-mediated oxidation purine decomposition products, including 7,8-dihydro-8-oxo-2'-deoxyguanosine and 7,8-dihydro-8-oxo-2'-deoxyadenosine, was searched within nucleosides and DNA by using specific and sensitive HPLC electrochemical assays. Emphasis was placed on the mechanistic aspects of the radical oxidation reactions involved in the formation of the two C(8) hydroxylated purine decomposition products.