An Enzyme-Responsive "Turn-on" Fluorescence Polymeric Superamphiphile as a Potential Visualizable Phosphate Prodrug Delivery Vehicle
An Enzyme-Responsive "Turn-on" Fluorescence Polymeric Superamphiphile as a Potential Visualizable Phosphate Prodrug Delivery Vehicle
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酶响应“开启”荧光聚合物超两亲物作为潜在的可视化磷酸盐前药输送载体
DOI：
10.1002/mabi.201800045
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发表时间：
2018
影响因子：
4.6
通讯作者：
Zhang Yanfeng
中科院分区：
文献类型：
--
作者：
Yang Xi;Shen Shihong;Guo Li;Tan Jidong;Lei Henxin;Wu Jianghan;Zhao Lei;Xiong Tao;Wu Youshen;Cheng Yilong;Zhang Yanfeng
廉价和高效的酶反应聚合物的发展为靶向药物递送系统做出了重大贡献。本文设计了一种具有荧光解离传感能力的超两亲体。它由带负电荷的5 ' -三磷酸腺苷（ATP）和带负电荷的二磷酸荧光素（FDP）组成，用于荧光检测，以及阳离子二嵌段共聚物甲氧基-聚（乙二醇）113 - b -聚（2 -二甲基-氨基乙基甲基丙烯酸酯）70。在添加犊牛肠道碱性磷酸酶后，超两亲体分解，可能是由于ATP的酶水解。这一过程伴随着荧光素荧光发射强度的增加，这是由于FDP的水解。超两亲体的体外应用也得到了证实。因此，超两亲体的“开启”荧光可作为检测超两亲体分解的实时模块。
The development of inexpensive and highly efficient enzyme‐responsive polymers has significantly contributed to targeted drug delivery systems. Here, a superamphiphile with a capability of fluorescent dissociation sensing is designed. It is constructed with negatively charged adenosine 5′‐triphosphate (ATP) and negatively charged fluorescein diphosphate (FDP), which are used as fluorescence detection, and a cationic diblock copolymer methoxy‐poly(ethylene glycol)113‐b‐poly(2‐dimethyl‐aminoethyl methacrylate)70. Upon addition of calf intestinal alkaline phosphatase, the superamphiphile disintegrates, presumably due to the enzymatic hydrolysis of ATP. This process is accompanied by an increase in the fluorescence emission intensity of fluorescein owing to the hydrolysis of FDP. The in vitro application of the superamphiphile is also proven. Thus, the “turn‐on” fluorescence of the superamphiphile serves as a real‐time module for detection of the disintegration of superamphiphile.