Isolation of a 3-hydroxypyridine degrading bacterium, Agrobacterium sp. DW-1, and its proposed degradation pathway
Isolation of a 3-hydroxypyridine degrading bacterium, Agrobacterium sp. DW-1, and its proposed degradation pathway
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3-羟基吡啶降解细菌农杆菌的分离。
DOI：
10.1186/s13568-019-0782-9
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发表时间：
2019-05
期刊：
影响因子：
3.7
通讯作者：
Yu Hao
中科院分区：
文献类型：
--
作者：
Zhao Shuxue;Hu Chunhui;Guo Lizhong;Li Kuiran;Yu Hao
从辽河流域石油污染土壤中分离到一株3-羟基吡啶降解菌，命名为DW-1。16 SrRNA系统发育分析表明菌株DW-1属于土壤杆菌属（Agrobacterium）。菌株DW-1产3-羟基吡啶的最适培养温度为30 °C，最适pH为8.0。在最佳条件下，DW-1在66 h内可完全降解1500 mg/L的3-羟基吡啶。通过HPLC和LC-MS分析，推测了菌株DW-1的3-羟基吡啶降解途径。菌株DW-1降解3-羟基吡啶的第一步反应是α-羟基化，生成主要代谢产物2，5-二羟基吡啶，然后2，5-二羟基吡啶被Fe 2+依赖的双加氧酶转化为N-甲酰马来酰胺酸。N-甲酰马来酰胺酸通过马来酰胺酸转化为马来酸和富马酸。这是第一次报道3-羟基吡啶的降解途径和利用3-羟基吡啶的土壤杆菌sp.它可能是潜在的用于生物修复的环境中3-羟基吡啶污染。
A 3-hydroxypyridine degrading bacterium, designated strain DW-1, was isolated from petroleum contaminated soil in Liao River China. 16S rRNA-based phylogenetic analysis indicates that strain DW-1 belongs to genus Agrobacterium. The optimal cultivation temperature and pH for strain DW-1 with 3-hydroxypyridine were 30 °C and 8.0, respectively. Under optimal conditions, strain DW-1 could completely degrade up to 1500 mg/L of 3-hydroxypyridine in 66 h. The 3-hydroxypyridine degradation pathway of strain DW-1 was suggested by HPLC and LC-MS analysis. The first reaction of 3-hydroxypyridine degradation in strain DW-1 was α-hydroxylation so that the major metabolite 2,5-dihydroxypyridine was produced, and then 2,5-dihydroxypyridine was transformed by a Fe2+-dependent dioxygenase to form N-formylmaleamic acid. N-Formylmaleamic acid will be transformed to maleic acid and fumaric acid through maleamic acid. This is the first report of the 3-hydroxypyridine degradation pathway and the utilization of 3-hydroxypyridine by a Agrobacterium sp. It may be potentially used for the bioremediation of environments polluted with 3-hydroxypyridine.
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