ACETYLCHOLINE RECEPTORS IN REGENERATING MUSCLE ACCUMULATE AT ORIGINAL SYNAPTIC SITES IN THE ABSENCE OF THE NERVE
ACETYLCHOLINE RECEPTORS IN REGENERATING MUSCLE ACCUMULATE AT ORIGINAL SYNAPTIC SITES IN THE ABSENCE OF THE NERVE
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DOI：
10.1083/jcb.82.2.412
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发表时间：
1979-01-01
影响因子：
7.8
通讯作者：
MCMAHAN, UJ
中科院分区：
文献类型：
--
作者：
BURDEN, SJ;SARGENT, PB;MCMAHAN, UJ
神经末梢在再生骨骼肌纤维乙酰胆碱受体组织化中的作用[R. pipiens]进行了检查。当肌纤维受损时，它们退化并被吞噬，但它们的基膜鞘存活。新的肌纤维在原始的基底层鞘内形成，并且它们精确地在鞘上的原始突触位点处受到神经支配。在去神经支配和损伤肌肉后，允许肌纤维再生，但故意阻止神经再支配。乙酰胆碱受体在再生肌纤维上的分布通过组织学方法测定，使用[125 I] α-银环蛇毒素或辣根过氧化物酶-α-银环蛇毒素;基底板鞘上的原始突触位点用胆碱酯酶染色标记。1个月。在肌肉损伤后，新的肌纤维具有选择性地定位于原始突触部位的乙酰胆碱受体的积累。这些部位的受体密度与正常神经肌肉接头相同。肌纤维表面的褶皱类似于正常神经肌肉接头处的接头褶皱，在没有神经末梢的情况下也发生在原始突触部位。再生肌肉中突触下膜的生物化学和结构组织由神经去除后保留在突触部位的结构指导。
The role of nerve terminals in organizing acetylcholine receptors on regenerating skeletal muscle fibers [R. pipiens] was examined. When muscle fibers are damaged, they degenerate and are phagocytized, but their basal lamina sheaths survive. New myofibers form within the original basal lamina sheaths, and they become innervated precisely at the original synaptic sites on the sheaths. After denervating and damaging muscle, myofibers were allowed to regenerate but deliberately prevented reinnervation. The distribution of acetylcholine receptors on regenerating myofibers was determined by histological methods, using [125I].alpha.-bungarotoxin or horseradish peroxidase-.alpha.-bungarotoxin; original synaptic sites on the basal lamina sheaths were marked by cholinesterase stain. By 1 mo. after damage to the muscle, the new myofibers have accumulations of acetylcholine receptors that are selectively localized to the original synaptic sites. The density of the receptors at these sites is the same as at normal neuromuscular junctions. Folds in the myofiber surface resembling junctional folds at normal neuromuscular junctions also occur at original synaptic sites in the absence of nerve terminals. The biochemical and structural organization of the subsynaptic membrane in regenerating muscle is directed by structures that remain at synaptic sites after removal of the nerve.