SPL5, a cell death and defense-related gene, encodes a putative splicing factor 3b subunit 3 (SF3b3) in rice
SPL5, a cell death and defense-related gene, encodes a putative splicing factor 3b subunit 3 (SF3b3) in rice
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SPL5 是一种细胞死亡和防御相关基因，编码水稻中假定的剪接因子 3b 亚基 3 (SF3b3)
DOI：
10.1007/s11032-011-9677-4
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发表时间：
2012-08-01
影响因子：
3.1
通讯作者：
Ma, Bojun
中科院分区：
文献类型：
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作者：
Chen, Xifeng;Hao, Liang;Ma, Bojun
水稻（Oryza sativa L.）斑点叶5（spl5）表明野生型SPL5负调节细胞死亡和抗性应答。此前，spl5基因已经通过图位克隆方法定位到两个标记SSR7和RM7121之间的80 kb区域。在这里，我们进一步表明，spl5基因被界定为一个15.1 kb的基因组区域的高分辨率序列靶位点（STS）标记。随后在该区域的spl5突变体测序显示，一个候选基因窝藏一个单碱基缺失，导致移码突变和提前终止密码子。生物信息学分析表明，SPL5基因编码一个推定的剪接因子3b亚基3（SF3b3），可能参与参与调节细胞死亡和抗性反应的未成熟RNA的剪接反应。进一步的分析表明野生型SPL5在功能上补充了spl5表型。本文提供的数据清楚地表明，SPL5通过调节植物中的RNA剪接来负调控细胞死亡和抗性反应。
A lesion-mimic phenotype in rice (Oryza sativa L.) spotted leaf 5 (spl5) indicates that wild-type SPL5 negatively regulates cell death and resistance responses. Previously, the spl5 gene was already mapped to the 80-kb region between two markers SSR7 and RM7121 through a map-based cloning approach. Here, we further showed that the spl5 gene was delimitated into a 15.1-kb genomic region by the high-resolution sequence target site (STS) markers. Subsequent sequencing in this region of spl5 mutant revealed that one candidate gene harbored a single-base deletion, resulting in a frame-shift mutation and a premature stop codon. Bioinformatic analysis showed that SPL5 gene encodes a putative splicing factor 3b subunit 3 (SF3b3) and might be involved in splicing reactions of pre-mature RNAs participating in the regulation of cell death and resistance responses. Further analysis showed that wild-type SPL5 did functionally complement the spl5 phenotype. The data presented here clearly indicate that the SPL5 negatively regulates cell death and resistance responses via modulating RNA splicing in plants.