Graphene oxide-enriched poly(ε-caprolactone) electrospun nanocomposite scaffold for bone tissue engineering applications
Graphene oxide-enriched poly(ε-caprolactone) electrospun nanocomposite scaffold for bone tissue engineering applications
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DOI：
10.1177/0883911516668666
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发表时间：
2017-05-01
影响因子：
1.7
通讯作者：
Solati-Hashjin, Mehran
中科院分区：
文献类型：
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作者：
Mohammadi, Sepideh;Shafiei, Seyedeh Sara;Solati-Hashjin, Mehran
组织工程旨在制造生物替代品来改善、修复和再生衰竭的人体组织或器官。设计具有定制特性的纳米复合材料支架可以促进功能组织的发展，是实现这一目标的适当途径。在本研究中，通过静电纺丝工艺，成功制备了不同氧化石墨烯纳米片含量（0.5 ~ 2wt%）的聚（ε -己内酯）纳米纤维，制备了均匀无珠的聚（ε -己内酯）纳米纤维。加入氧化石墨烯纳米片后，聚（ε -己内酯）纳米纤维的平均直径减小。此外，与纯聚-己内酯相比，含有2 wt%氧化石墨烯纳米片的纳米复合支架表现出优越的机械性能。与纯聚（epsilon-己内酯）支架相比，聚（epsilon-己内酯）-氧化石墨烯纳米片纳米纤维的降解率提高，同时保持了纤维的完整性。聚（ε -己内酯）纤维中氧化石墨烯纳米片的存在促进了体外生物矿化，表明了纳米复合支架的生物活性特征。与纯纤维相比，纳米复合纤维也表现出更好的蛋白质吸附能力。体外细胞培养研究表明，氧化石墨烯纳米片的加入并未降低静电纺聚（epsilon-己内酯）纳米纤维的生物相容性。此外，MG63细胞的黏附和增殖均有明显增强。总之，结果表明，静电纺聚（ε -己内酯）-氧化石墨烯纳米片纳米纤维可能是组织工程支架应用的合适候选材料。
Tissue engineering aims at fabricating biological substitutes to improve, repair, and regenerate failing human tissues or organs. Designing a nanocomposite scaffolds with tailored properties that enhance the development of functional tissue can be an appropriate approach to achieve this purpose. In this study, the uniform and bead-free nanofibers of poly(epsilon-caprolactone) composited with different graphene oxide nanosheet contents (ranging from 0.5 to 2wt%) were successfully fabricated through electrospinning process. A decrease in the average diameter of poly(epsilon-caprolactone) nanofibers was observed with the addition of graphene oxide nanosheets. Moreover, the nanocomposite scaffolds containing 2 wt% of graphene oxide nanosheets exhibited superior mechanical properties compared to that of pure poly(epsilon-caprolactone). Compared with pure poly(epsilon-caprolactone) scaffold, the degradation rate of poly(epsilon-caprolactone)-graphene oxide nanosheet nanofibers was enhanced, while the integrity of fibers was preserved. The presence of graphene oxide nanosheets in poly(epsilon-caprolactone) fibers promoted in vitro biomineralization, indicating bioactive features of the nanocomposite scaffolds. Compared to the pure one, nanocomposite fibers also showed better ability in protein adsorption. The in vitro cell culture studies showed that the addition of graphene oxide nanosheets did not diminish the biocompatibility of the electrospun poly(epsilon-caprolactone) nanofiber. Furthermore, the adhesion and proliferation of MG63 cells were increased. Altogether, the results demonstrated that electrospun poly(epsilon-caprolactone)-graphene oxide nanosheet nanofiber may be a suitable candidate for tissue engineering scaffold applications.