Quinine inhibits multiple Na+ and K+ transport mechanisms in Ehrlich ascites tumor cells.
Quinine inhibits multiple Na+ and K+ transport mechanisms in Ehrlich ascites tumor cells.
复制标题
奎宁抑制艾利希腹水肿瘤细胞中的多种钠和钾转运机制。
DOI：
10.1016/0005-2736(89)90512-9
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Levinson,C
中科院分区：
文献类型：
--
作者：
Smith,TC;Levinson,C
在艾氏腹水癌细胞中，研究了奎宁与K+、Na+转运机制的相互作用。奎宁对钙依赖的K+通道和总K+内流均有影响。心得安对钙依赖性钾通道的激活作用可被奎宁(1 MM)所阻断。此外，奎宁抑制哇巴因敏感组分的K+内流，其KI为0.32±0.02 mM，抑制速尿敏感组分的KI为0.24±0.01 mM。此外，很大一部分(52%)的Na+内流被奎宁抑制。同一组分对阿米洛利敏感，提示其代表Na+/H+逆向转运。伴随着K+和Na+转运的抑制，奎宁能刺激57%的ATP水解率。结果表明，奎宁对调节Ehrlich细胞阳离子转运的细胞机制具有广泛的、非特异性的影响。
The interaction of quinine with K+and Na+transport mechanisms has been investigated in Ehrlich ascites tumor cells. Quinine affects both Ca2+-dependent K+channel and total K+influx. Activation of Ca+-dependent K+channels by propranolol is abolished by quinine (1 mM). In addition, quinine inhibits the ouabain-sensitive component of K+influx with an apparentKiof 0.32 ± 0.02 mM and the furosemide-sensitive component with aKiof 0.24 ± 0.01 mM. Furthermore, a significant fraction (52%) of Na+influx is inhibited by quinine. The same component is sensitive to amiloride, suggesting that it represents Na+/H+antiport. Concomitant with the inhibition of K+and Na+transport, quinine stimulates ATP hydrolysis by 57%. The results suggest that quinine exerts broad, nonspecific effects on cellular mechanisms which serve to regulate cation transport in Ehrlich cells.