The resolution of bimodal peaks in the analytical ultracentrifuge in the presence and absence of ligands.
The resolution of bimodal peaks in the analytical ultracentrifuge in the presence and absence of ligands.
复制标题
在存在和不存在配体的情况下,分析超速离心机中双峰的分辨率。
DOI:
10.1016/0003-2697(83)90159-8
复制
发表时间:
1983
影响因子:
2.9
通讯作者:
Timasheff,SN
中科院分区:
文献类型:
--
作者:
Prakash,V;Timasheff,SN
The patterns for peak resolution in the analytical ultracentrifuge characteristic of systems associating by different mechanisms are analyzed. These are (i) that in which reequilibration is infinitely slow; (ii) that in which reequilibration is rapid, i.e., a Gilbert-type system; and (iii) that in which reequilibration is rapid, but the self-association is mediated by a ligand. Criteria for distinguishing the three are described and illustrated by results on tubulin self-association under various conditions.