Fabrication of sustained-release zein nanoparticles via modified coaxial electrospraying
Fabrication of sustained-release zein nanoparticles via modified coaxial electrospraying
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DOI：
10.1016/j.cej.2017.10.098
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发表时间：
2018-02-15
影响因子：
15.1
通讯作者：
Li, Hao-Lin
中科院分区：
文献类型：
--
作者：
Liu, Zhe-Peng;Zhang, Yao-Yao;Li, Hao-Lin
一种改进的同轴电喷雾过程，其特征在于使用纯溶剂作为鞘工作流体，被开发用于药物纳米颗粒的连续和稳健的生成。柠檬酸他莫昔芬（TAM）被封装在玉米醇溶蛋白，一种植物蛋白。详细研究了关键参数壳-核流体流速对电喷雾过程和所得蛋白质基复合颗粒的影响。扫描电子显微镜图像显示，与传统的单流体法相比，改进的同轴法制备的玉米醇溶蛋白颗粒更圆、粒径更小、粒径分布更窄、内部结构更致密。由于TAM和玉米醇溶蛋白之间的次级相互作用增强，所有颗粒都是类似的无定形复合物，如通过它们的X射线图案和衰减全反射傅立叶变换红外光谱所验证的。与传统的单流体法相比，同轴法制备的药物纳米粒具有更好的药物缓释特性，初始突释量更小，释药时间更长，拖尾时间更短。提出了电喷雾微粒的微观形成机制及其药物缓释机制。通过改变传统的电喷雾的概念，改进的同轴电喷雾方法在这项研究中开发了一个强大的新的过程中产生的纳米粒子具有新颖的结构和功能。
A modified coaxial electrospraying process, which was characterized by the use of pure solvent as a sheath working fluid, was developed for the continuous and robust generation of medicated nanoparticles. Tamoxifen citrate (TAM) was encapsulated in zein, a plant protein. The influences of the key parameter shell-to-core fluid flow rate on the electrospraying processes and on the resulting protein-based composite particles were investigated in detail. Scanning electron microscopy images revealed that compared with particles prepared via the traditional single-fluid process, TAM-loaded zein particles prepared via the modified coaxial process were rounder with smaller diameters, narrower smaller size distribution, and more compact inner structures. Owing to the reinforced secondary interactions between TAM and zein, all the particles were similarly amorphous composites, as verified by their X-ray patterns and attenuated total reflectance-Fourier transform infrared spectra. The medicated nanoparticles synthesized via the coaxial process exhibited a better drug sustained-release profile than those synthesized via the traditional single-fluid process, with a smaller initial burst release, longer release period, and shorter tailing-off stage. The microformation mechanisms of the electrosprayed particles and their drug sustained-release mechanisms were suggested. By altering the traditional concept of electrospraying, the modified coaxial electrospraying method developed in this study provides a powerful new process for the generation of nanoparticles with novel structures and functions.