Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4
Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4
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DOI：
10.1093/emboj/20.19.5400
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发表时间：
2001-10-01
期刊：
影响因子：
11.4
通讯作者：
Parker, JE
中科院分区：
文献类型：
--
作者：
Feys, BJ;Moisan, LJ;Parker, JE
拟南芥EDS 1和PAD 4基因编码在抗性（R）基因介导的和基础植物疾病抗性中起作用的脂肪酶样蛋白。edS 1和pad 4无效突变体的表型分析表明，edS 1和pad 4是由相同谱的R基因调节的抗性所需的，但在防御途径中发挥不同的作用。EDS 1对于植物过敏反应的产生是必不可少的，而EDS 1和PAD 4都是植物防御增强分子水杨酸积累所必需的。EDS 1是病原体诱导的PAD 4 mRNA积累所必需的，而PAD 4突变或水杨酸耗竭仅部分损害EDS 1表达。酵母双杂交分析表明，EDS 1可以二聚化并与PAD 4相互作用。然而，EDS 1二聚化由与参与EDS 1-PAD 4缔合的结构域不同的结构域介导。免疫共沉淀实验表明，EDS 1和PAD 4蛋白在健康和病原体攻击的植物细胞中相互作用。我们为EDS 1提出了两个功能。第一个是在植物防御早期所需的，独立于PAD 4。第二个招募PAD 4在扩增的防御，可能是通过直接的EDS 1-PAD 4协会。
The Arabidopsis EDS1 and PAD4 genes encode lipase-like proteins that function in resistance (R) gene-mediated and basal plant disease resistance. Phenotypic analysis of eds] and pad4 null mutants shows that EDS1 and PAD4 are required for resistance conditioned by the same spectrum of R genes but fulfil distinct roles within the defence pathway. EDS1 is essential for elaboration of the plant hypersensitive response, whereas EDS1 and PAD4 are both required for accumulation of the plant defence-potentiating molecule, salicylic acid. EDS1 is necessary for pathogen-induced PAD4 mRNA accumulation, whereas mutations in PAD4 or depletion of salicylic acid only partially compromise EDS1 expression. Yeast two-hybrid analysis reveals that EDS1 can dimerize and interact with PAD4. However, EDS1 dimerization is mediated by different domains to those involved in EDS1-PAD4 association. Co-immunoprecipitation experiments show that EDS1 and PAD4 proteins interact in healthy and pathogen-challenged plant cells. We propose two functions for EDS1. The first is required early in plant defence, independently of PAD4. The second recruits PAD4 in the amplification of defences, possibly by direct EDS1-PAD4 association.