Super-resolution fluorescence microscopy.

Super-resolution fluorescence microscopy.
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DOI:
10.1146/annurev.biochem.77.061906.092014
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发表时间:
2009
影响因子:
16.6
通讯作者:
Zhuang X
Zhuang X
中科院分区:
生物学1区
文献类型:
--
作者:
Huang B;Bates M;Zhuang X

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实现的空间分辨率,不受光的衍射限制,超分辨率荧光显微镜技术的最新发展允许许多生物结构的观察在传统的荧光显微镜中无法分辨。这些技术的新进展现在使他们能够对三维(3D)结构进行成像,通过微共定位测量相互作用,并在纳米尺度上记录活细胞中的动态过程。预计超分辨率荧光显微镜将成为细胞和组织成像的广泛使用的工具,以提供以前未观察到的生物结构和过程的细节。
Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional fluorescence microscopy. New advances in these techniques now give them the ability to image three-dimensional (3D) structures, measure interactions by multicolor colocalization, and record dynamic processes in living cells at the nanometer scale. It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes.
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